PMID- 10763585
OWN - NLM
STAT- MEDLINE
DCOM- 20000518
LR  - 20220223
IS  - 0032-0943 (Print)
IS  - 0032-0943 (Linking)
VI  - 66
IP  - 2
DP  - 2000 Mar
TI  - Hepatoprotective effect of Apocynum venetum and its active constituents.
PG  - 127-33
AB  - The leaves of Apocynum venetum L. are used as a tea material in north China and 
      Japan. A water extract (500 mg/kg/day, one week administration) of the leaves of 
      A. venetum showed protective effects against carbon tetrachloride (CCl4, 30 
      microliters/mouse) or D-galactosamine (D-GalN, 700 mg/kg)/lipopolysaccharide 
      (LPS, 20 micrograms/kg)-induced liver injury in mice. Tumor necrosis factor-alpha 
      (TNF-alpha) secreted from LPS-stimulated macrophages is the most crucial mediator 
      in the D-GalN/LPS-induced liver injury model. The extract had no significant 
      inhibition on the increase of serum TNF-alpha (1169 +/- 132 pg/ml vs. 1595 +/- 
      314 pg/ml of control), but exhibited a complete inhibition at the concentration 
      of 100 micrograms/ml on TNF-alpha (100 ng/ml)-induced cell death in D-GalN (0.5 
      mM)-sensitized mouse hepatocytes. Further activity-guided fractionation resulted 
      in the isolation of fifteen flavonoids viz. (-)-epicatechin (1), 
      (-)-epigallocatechin (2), isoquercetin (3), hyperin (4), (+)-catechin (5), 
      (+)-gallocatechin (6), kaempferol-6'-O-acetate (7), isoquercetin-6'-O-acetate 
      (8), catechin-[8,7-e]-4 alpha-(3,4-dihydroxpyhenyl)-dihydro-2(3H)-pyranone (9), 
      apocynin B (10), apocynin A (11), cinchonain Ia (12), apocynin C (13), apocynin D 
      (14) and quercetin (15). All the compounds showed inhibitory effects on 
      TNF-alpha-induced cell death with different intensities. The flavonol glycosides 
      3, 4, 7 and 8 and the phenylpropanoid-substituted flavan-3-ols 11 and 12 showed 
      potent inhibitory effects on TNF-alpha-induced cell death with IC50 values of 
      37.5, 14.5, 31.2, 55.1, 71.9 and 41.2 microM, respectively. In contrast, the 
      clinically used 5 and its analogues 1, 2 and 6 showed apparent activity only at 
      80 microM. These flavonoids appeared to be the hepatoprotective principles of the 
      leaves of A. venetum. The hepatoprotective effects exhibited by the extract and 
      its constituents suggest a validation of the leaves as a tea material.
FAU - Xiong, Q
AU  - Xiong Q
AD  - Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 
      Japan.
FAU - Fan, W
AU  - Fan W
FAU - Tezuka, Y
AU  - Tezuka Y
FAU - Adnyana, I K
AU  - Adnyana IK
FAU - Stampoulis, P
AU  - Stampoulis P
FAU - Hattori, M
AU  - Hattori M
FAU - Namba, T
AU  - Namba T
FAU - Kadota, S
AU  - Kadota S
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Planta Med
JT  - Planta medica
JID - 0066751
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
SB  - IM
MH  - Animals
MH  - Carbon Tetrachloride/toxicity
MH  - Cell Death/drug effects
MH  - Liver/cytology/*drug effects
MH  - Male
MH  - Mice
MH  - Plant Extracts/*pharmacology
MH  - Plants, Medicinal/*chemistry
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2000/04/14 09:00
MHDA- 2000/05/20 09:00
CRDT- 2000/04/14 09:00
PHST- 2000/04/14 09:00 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 2000/04/14 09:00 [entrez]
AID - 10.1055/s-2000-11135 [doi]
PST - ppublish
SO  - Planta Med. 2000 Mar;66(2):127-33. doi: 10.1055/s-2000-11135.