PMID- 10764820
OWN - NLM
STAT- MEDLINE
DCOM- 20000816
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 25
DP  - 2000 Jun 23
TI  - Tumor necrosis factor-alpha production is differently regulated in gamma delta 
      and alpha beta human T lymphocytes.
PG  - 19282-7
AB  - Tumor necrosis factor-alpha (TNF-alpha) plays a crucial role in the early defense 
      against pathogens. This cytokine is produced by several cell types including T 
      lymphocytes expressing the alphabeta as well as the gammadelta T cell receptor 
      (TcR). In human, the circulating gammadelta T cells, which mostly express 
      Vgamma9Vdelta2 TcR, have been strongly suggested to play an important protective 
      role against infectious agents. These activated cells early produce high amounts 
      of TNF-alpha, which induce a determinant beneficial effect against development of 
      intracellular pathogens; however, sustained production of this cytokine can 
      result in immunopathological diseases. The signals that regulate TNF-alpha 
      production in Vgamma9Vdelta2 T cells are totally unknown. In primary alphabeta T 
      cells, TNF-alpha production was shown to necessitate engagement of the TcR and 
      CD28, and to be independent of the p38 mitogen activated protein kinase pathway. 
      We demonstrate herein that, in contrast to alphabeta T cells, TNF-alpha 
      production in Vgamma9Vdelta2 T lymphocytes is independent of CD28 costimulation 
      and highly dependent on TcR-induced p38 kinase and extracellular signal-regulated 
      kinase 2 pathway activation for optimal cytokine release. Moreover, we bring 
      elements supporting the idea that the "activation threshold" of gammadelta T 
      cells leading to cytokine production is lower than that of alphabeta T cells.
FAU - Lafont, V
AU  - Lafont V
AD  - INSERM U431, Microbiologie et Pathologie Cellulaire Infectieuse, Universite 
      Montpellier 2, Place Eugene Bataillon, cc 100, 34095 Montpellier cedex 05, 
      France.
FAU - Liautard, J
AU  - Liautard J
FAU - Gross, A
AU  - Gross A
FAU - Liautard, J P
AU  - Liautard JP
FAU - Favero, J
AU  - Favero J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CD28 Antigens)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 0 (Receptors, Antigen, T-Cell, gamma-delta)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - CD28 Antigens/immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Receptors, Antigen, T-Cell, alpha-beta/*immunology
MH  - Receptors, Antigen, T-Cell, gamma-delta/*immunology
MH  - Signal Transduction
MH  - T-Lymphocytes/immunology/*metabolism
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 2000/04/15 09:00
MHDA- 2000/08/19 11:00
CRDT- 2000/04/15 09:00
PHST- 2000/04/15 09:00 [pubmed]
PHST- 2000/08/19 11:00 [medline]
PHST- 2000/04/15 09:00 [entrez]
AID - S0021-9258(19)80196-5 [pii]
AID - 10.1074/jbc.M910487199 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jun 23;275(25):19282-7. doi: 10.1074/jbc.M910487199.