PMID- 10765058
OWN - NLM
STAT- MEDLINE
DCOM- 20000606
LR  - 20171101
IS  - 0014-3022 (Print)
IS  - 0014-3022 (Linking)
VI  - 43
IP  - 3
DP  - 2000
TI  - Detection of human herpesvirus 6 variant A in peripheral blood mononuclear cells 
      from multiple sclerosis patients.
PG  - 170-3
AB  - Several authors report that human herpesvirus 6 (HHV-6) variants have different 
      epidemiologies, in vivo tropism and pathogenic potentials. However, it is not 
      well known what pathogenic roles its neurotropism might have in the variant type. 
      As some active plaques of multiple sclerosis (MS) brain tissue harbor HHV-6 DNA 
      divergent from the prototype virus, the possibility that the variant strain may 
      play a role in the pathogenesis of MS has been suggested. Therefore, we tried to 
      investigate the role of HHV-6 variants in the pathogenesis of MS. As HHV-6 is 
      predominantly a T-cell-tropic virus, we examined HHV-6 DNA sequences in 
      peripheral blood mononuclear cells (PBMC) from 34 MS patients, 6 with idiopathic 
      transverse myelitis, 2 with optic neuritis and 20 healthy controls. Nested 
      polymerase chain reaction was used to detect the HHV-6 genome. To discern HHV-6 
      variants A and B, amplification products were digested by restriction enzyme. We 
      found that 7 of 34 MS patients and 2 of 6 patients with idiopathic transverse 
      myelitis had the HHV-6 genome. On the contrary, there was no HHV-6 genome in the 
      control group. All genomic sequences were of HHV-6 variant A (HHV-6A). Our 
      results suggest that the detection of HHV-6A in the PBMC of patients with MS may 
      raise the possibility of a relationship between latent HHV-6A infection and the 
      pathogenesis of MS.
CI  - Copyright 2000 S. Karger AG, Basel
FAU - Kim, J S
AU  - Kim JS
AD  - Department of Neurology, Catholic University of Korea, Seoul, South Korea.
FAU - Lee, K S
AU  - Lee KS
FAU - Park, J H
AU  - Park JH
FAU - Kim, M Y
AU  - Kim MY
FAU - Shin, W S
AU  - Shin WS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Eur Neurol
JT  - European neurology
JID - 0150760
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - DNA, Viral/analysis
MH  - Female
MH  - Herpesvirus 6, Human/*isolation & purification/pathogenicity
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*virology
MH  - Multiple Sclerosis/*virology
MH  - Myelitis, Transverse/virology
MH  - Polymerase Chain Reaction
MH  - Virulence
EDAT- 2000/04/15 09:00
MHDA- 2000/06/10 09:00
CRDT- 2000/04/15 09:00
PHST- 2000/04/15 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/04/15 09:00 [entrez]
AID - 8158 [pii]
AID - 10.1159/000008158 [doi]
PST - ppublish
SO  - Eur Neurol. 2000;43(3):170-3. doi: 10.1159/000008158.