PMID- 10768731
OWN - NLM
STAT- MEDLINE
DCOM- 20000629
LR  - 20191103
IS  - 0905-6157 (Print)
IS  - 0905-6157 (Linking)
VI  - 11
IP  - 1
DP  - 2000 Feb
TI  - Determination of T-cell subpopulations and intracellular cytokine production 
      (interleukin-2, interleukin-4, and interferon-gamma) by cord blood T-lymphocytes 
      of neonates from atopic and non-atopic parents.
PG  - 12-9
AB  - This report describes the results of a prospective study on immunological markers 
      in cord blood for the prediction of allergic diseases in children. First we 
      evaluated methodological aspects of the flow cytometric technique on cord blood 
      cytokine measurements. Subsequently, the T-cell subsets and percentage of 
      cytokine-producing cord blood T-helper (Th) and T-suppressor/cytotoxic 
      lymphocytes of neonates from atopic and non-atopic parents were compared. A group 
      of 33 healthy, full-term newborn infants of whom 23/33 were at risk for atopy 
      (i.e. having at least one parent with one or more atopic symptoms and positive 
      specific immunoglobulin E [IgE] to at least one common inhalant allergen) was 
      studied. A flow cytometric technique was used to analyze cord blood T-cell 
      subsets and to determine the percentage of interleukin (IL)-2-, IL-4-, and 
      interferon-gamma (IFN-gamma)-producing cord blood Th and T-suppressor/cytotoxic 
      lymphocytes following stimulation with phorbol 12-myristate 13-acetate (PMA) and 
      ionomycin. The percentage of CD3 (T lymphocytes), CD3+ CD4+ (Th lymphocytes), 
      CD3+ CD8+ (T-suppressor/cytotoxic lymphocytes), CD19+ (B lymphocytes), CD3+ CD4+ 
      CD45RO+ (memory Th lymphocytes), and CD3+ CD4+ CD45RA+ (naive Th lymphocytes) 
      cells was unrelated to parental atopic status. PMA stimulation augmented the 
      percentage of IL-2- and IFN-gamma-producing Th and T-suppressor/cytotoxic 
      lymphocytes, whereas the number of IL-4-producing T lymphocytes remained very low 
      or undetectable. No differences in the percentage of TL-2-, IL-4- and 
      IFN-gamma-producing Th and T-suppressor/cytotoxic lymphocytes were found between 
      neonates from atopic and non-atopic parents. These results will be re-evaluated 
      when the atopic status of the children at the age of 1 and 2 years can be 
      assessed.
FAU - Hagendorens, M M
AU  - Hagendorens MM
AD  - Department of Paediatrics, University of Antwerp, UIA, Belgium.
FAU - Van Bever, H P
AU  - Van Bever HP
FAU - Schuerwegh, A J
AU  - Schuerwegh AJ
FAU - De Clerck, L S
AU  - De Clerck LS
FAU - Bridts, C H
AU  - Bridts CH
FAU - Stevens, W J
AU  - Stevens WJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Pediatr Allergy Immunol
JT  - Pediatric allergy and immunology : official publication of the European Society 
      of Pediatric Allergy and Immunology
JID - 9106718
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-2)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
CIN - Pediatr Allergy Immunol. 2001 Aug;12(4):231-2. PMID: 11555321
MH  - Animals
MH  - Blood Preservation
MH  - Cell Separation
MH  - Cytokines/*biosynthesis/blood
MH  - Eosinophils/cytology
MH  - Female
MH  - Fetal Blood/cytology/*immunology/*metabolism
MH  - Humans
MH  - Hypersensitivity, Immediate/blood/*immunology
MH  - Infant, Newborn
MH  - Interferon-gamma/biosynthesis/blood
MH  - Interleukin-2/biosynthesis/blood
MH  - Interleukin-4/biosynthesis/blood
MH  - Leukocyte Count
MH  - Lymphocyte Count
MH  - Male
MH  - Observer Variation
MH  - Prospective Studies
MH  - T-Lymphocyte Subsets/cytology/*immunology/*metabolism
EDAT- 2000/04/18 09:00
MHDA- 2000/07/06 11:00
CRDT- 2000/04/18 09:00
PHST- 2000/04/18 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/04/18 09:00 [entrez]
AID - 10.1034/j.1399-3038.2000.00054.x [doi]
PST - ppublish
SO  - Pediatr Allergy Immunol. 2000 Feb;11(1):12-9. doi: 
      10.1034/j.1399-3038.2000.00054.x.