PMID- 10771106
OWN - NLM
STAT- MEDLINE
DCOM- 20000607
LR  - 20190915
IS  - 0168-0102 (Print)
IS  - 0168-0102 (Linking)
VI  - 36
IP  - 4
DP  - 2000 Apr
TI  - Evidence of [Ca(2+)]i elevation by anti-calreticulin immunoreactive protein in 
      neurons.
PG  - 285-90
AB  - Calreticulin is a multifunctional Ca(2+)-binding protein. The effect of 
      anti-calreticulin antibody on intracellular free calcium concentration [Ca(2+)]i 
      was studied in cultured neurons using fura-2 based microfluorometry. 
      Anti-calreticulin increased [Ca(2+)]i in a dose dependent manner. The 
      anti-calreticulin antibody produced a rapid transient [Ca(2+)]i peak followed by 
      a long slowly decaying plateau. Anti-calreticulin induced extracellular Ca(2+) 
      influx in cultured neuron cells was blocked partially by N-methyl-D-aspartate 
      receptor (NMDAR) antagonist 2-amino-5-phosphonovaleric acid (AP5) and spider 
      polyamine toxin JSTX-3, which is recognized as a blocker of glutamatergic nervous 
      system. Furthermore, anti-calreticulin induced intracellular Ca(2+) desensitized 
      NMDAR. Dual immunofluorescent staining studies revealed that NMDAR co-localized 
      with calreticulin in the cultured neurons. Thus, the signal transduction system 
      of NMDA might be closely concerned with the extracellular calreticulin like 
      protein.
FAU - Hossain, M A
AU  - Hossain MA
AD  - Suntory Institute for Bioorganic Research, 1-1-1 Wakayamadai, Shimamotocho, 
      Mishimagun, Osaka, Japan. hossain@sunbor.or.jp
FAU - Murayama, N
AU  - Murayama N
FAU - Oka, T
AU  - Oka T
FAU - Nakajima, T
AU  - Nakajima T
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Calreticulin)
RN  - 0 (Ribonucleoproteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amphibians
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium-Binding Proteins/*analysis
MH  - Calreticulin
MH  - Cells, Cultured
MH  - Embryo, Mammalian
MH  - Embryo, Nonmammalian
MH  - Humans
MH  - Immunohistochemistry
MH  - Neurons/cytology/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Ribonucleoproteins/*analysis
EDAT- 2000/04/20 09:00
MHDA- 2000/06/10 09:00
CRDT- 2000/04/20 09:00
PHST- 2000/04/20 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/04/20 09:00 [entrez]
AID - S0168010299001303 [pii]
AID - 10.1016/s0168-0102(99)00130-3 [doi]
PST - ppublish
SO  - Neurosci Res. 2000 Apr;36(4):285-90. doi: 10.1016/s0168-0102(99)00130-3.