PMID- 10771373
OWN - NLM
STAT- MEDLINE
DCOM- 20000810
LR  - 20191103
IS  - 0955-3886 (Print)
IS  - 0955-3886 (Linking)
VI  - 22
IP  - 1-2
DP  - 2000 Feb-Apr
TI  - In vitro analysis of platelet concentrates stored in the presence of modulators 
      of 3',5' adenosine monophosphate, and organic anions.
PG  - 3-11
AB  - The storage of conventional platelet concentrates (PCs) under standard blood bank 
      conditions is limited to five days, in part because longer storage periods lead 
      to increasing damage in platelet integrity and functionality. The growing demand 
      of PCs for clinical use, raises the interest to develop agents that would 
      potentially permit a more extended period of storage. We have evaluated and 
      compared the in vitro quality of PCs treated with: (1) Modulators of levels of 
      cAMP (PGE1, foskolin, theophylline and isobutyl-methyl-xanthine [IBMX]); and (2) 
      organic anions that function as alternative substrates of platelets (pyruvate and 
      acetate). Platelet rich plasma (PRP) from pools (n = 6) of PCs was distributed 
      into storage bags, and the agents to be tested were added, using saline as a 
      control substance. PCs were stored at 22 degrees C with continuous agitation for 
      up to 10 days. At 0, 5 and 10 days of storage, samples were analyzed for platelet 
      counts, mean platelet volume (MPV), metabolic markers, and expression of 
      glycoproteins (GPs). The addition of modulators of levels of cAMP, at the 
      concentration used in the study, did not lead to substantial improvement in the 
      parameters being evaluated, with respect to those in control units. The 
      supplementation with organic anions, while not affecting the surface levels of 
      GPs, favored the maintenance of metabolic values, such as pH, PCO2, and 
      bicarbonate concentrations, as well as the preservation of MPV (p values < 0.05 
      respect to control units both at 5 and 10 days of storage). Our results indicate 
      that while the use of modulators of levels of cAMP do not provide substantial 
      benefit in the prevention of platelet storage lesions, organic anions have some 
      advantageous effect in the storage promoted metabolic changes of PCs. These data 
      might be considered when designing strategies to improve PC storage.
FAU - Lozano, M L
AU  - Lozano ML
AD  - Unit of Oncohematology and Hemotherapy, University General Hospital, School of 
      Medicine, Murcia, Spain.
FAU - Rivera, J
AU  - Rivera J
FAU - Bermejo, E
AU  - Bermejo E
FAU - Corral, J
AU  - Corral J
FAU - Perez, E
AU  - Perez E
FAU - Vicente, V
AU  - Vicente V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Transfus Sci
JT  - Transfusion science
JID - 9001514
RN  - 0 (Antigens, CD)
RN  - 0 (CD63 protein, human)
RN  - 0 (P-Selectin)
RN  - 0 (Platelet Glycoprotein GPIIb-IIIa Complex)
RN  - 0 (Platelet Glycoprotein GPIb-IX Complex)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Tetraspanin 30)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 1F7A44V6OU (Colforsin)
RN  - 8558G7RUTR (Pyruvic Acid)
RN  - 8MDF5V39QO (Sodium Bicarbonate)
RN  - C137DTR5RG (Theophylline)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - F5TD010360 (Alprostadil)
RN  - Q40Q9N063P (Acetic Acid)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - Acetic Acid/pharmacology
MH  - Alprostadil/pharmacology
MH  - Antigens, CD/drug effects
MH  - *Blood Platelets/drug effects/metabolism
MH  - *Blood Preservation
MH  - Carbon Dioxide/blood
MH  - Cell Count
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/*blood/metabolism/pharmacology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - L-Lactate Dehydrogenase
MH  - P-Selectin/blood/drug effects
MH  - Platelet Activation/drug effects/immunology
MH  - Platelet Count/drug effects
MH  - Platelet Glycoprotein GPIIb-IIIa Complex
MH  - Platelet Glycoprotein GPIb-IX Complex
MH  - Platelet Membrane Glycoproteins/drug effects
MH  - Pyruvic Acid/pharmacology
MH  - Sodium Bicarbonate/blood
MH  - Tetraspanin 30
MH  - Theophylline/pharmacology
MH  - Time Factors
EDAT- 2000/04/20 09:00
MHDA- 2000/09/08 11:01
CRDT- 2000/04/20 09:00
PHST- 2000/04/20 09:00 [pubmed]
PHST- 2000/09/08 11:01 [medline]
PHST- 2000/04/20 09:00 [entrez]
AID - S0955-3886(00)00002-3 [pii]
AID - 10.1016/s0955-3886(00)00002-3 [doi]
PST - ppublish
SO  - Transfus Sci. 2000 Feb-Apr;22(1-2):3-11. doi: 10.1016/s0955-3886(00)00002-3.