PMID- 10772304
OWN - NLM
STAT- MEDLINE
DCOM- 20000505
LR  - 20190826
IS  - 0886-4470 (Print)
IS  - 0886-4470 (Linking)
VI  - 126
IP  - 4
DP  - 2000 Apr
TI  - Delayed repair of transected nerves: effect of brain-derived neurotrophic factor.
PG  - 501-5
AB  - OBJECTIVE: To determine if administration of brain-derived neurotrophic factor 
      (BDNF) after peripheral nerve transection can improve the functional outcome in 
      situations where epineurial repair must be delayed. DESIGN: Randomized, blinded, 
      controlled trial. SUBJECTS: Thirty-four Sprague-Dawley rats. INTERVENTION: 
      Sciatic nerves were transected and, after a 2-week delay, repaired with 
      epineurial sutures. Animals were assigned to receive daily administration of 
      lactated Ringer solution (LR [control] group); BDNF delivered at the time of 
      nerve transection through 2 weeks after nerve repair, for a total of 4 weeks 
      (BDNF-early group); or BDNF delivered at the time of nerve repair through 2 weeks 
      after repair (BDNF-late group). Outcome was assessed using sciatic functional 
      indices (SFIs) and histomorphometric analysis. RESULTS: The SFI maximal recovery 
      was superior in the BDNF groups, but this difference did not reach statistical 
      significance (SFI, -90.1+/-9.6 [LR group], -85.7+/-7.6 [BDNF-early group], and 
      -84.6+/-4.8 [BDNF-late group], where normal function is 0 and complete loss of 
      function is -100; P = .27). The mean axon diameter tended to be greater in the 
      BDNF groups compared with the LR group, i.e., 2.43+/-0.23 microm (LR group), 
      2.80+/-0.44 microm (BDNF-early group), and 2.83+/-0.38 microm (BDNF-late group) 
      (P = .05). CONCLUSIONS: The local administration of BDNF to nerves that underwent 
      transection and then repair after a delay resulted in an increase in axonal 
      diameters and maximal SFIs, a difference that did not reach statistical 
      significance. The timing of BDNF administration after nerve transection did not 
      affect neuronal regeneration.
FAU - Moir, M S
AU  - Moir MS
AD  - Division of Otolaryngology-Head and Neck Surgery, Stanford University Medical 
      Center, Calif., USA.
FAU - Wang, M Z
AU  - Wang MZ
FAU - To, M
AU  - To M
FAU - Lum, J
AU  - Lum J
FAU - Terris, D J
AU  - Terris DJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Arch Otolaryngol Head Neck Surg
JT  - Archives of otolaryngology--head & neck surgery
JID - 8603209
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Axons/physiology
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Male
MH  - Nerve Regeneration/*drug effects
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/anatomy & histology/*physiology
MH  - Suture Techniques
MH  - Time Factors
EDAT- 2000/04/20 09:00
MHDA- 2000/05/16 09:00
CRDT- 2000/04/20 09:00
PHST- 2000/04/20 09:00 [pubmed]
PHST- 2000/05/16 09:00 [medline]
PHST- 2000/04/20 09:00 [entrez]
AID - 10.1001/archotol.126.4.501 [doi]
PST - ppublish
SO  - Arch Otolaryngol Head Neck Surg. 2000 Apr;126(4):501-5. doi: 
      10.1001/archotol.126.4.501.