PMID- 10772814
OWN - NLM
STAT- MEDLINE
DCOM- 20000518
LR  - 20121115
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 256
IP  - 2
DP  - 2000 May 1
TI  - Requirement of regulated activation of Ras for response of MDCK cells to 
      hepatocyte growth factor/scatter factor.
PG  - 411-22
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) induces cell scattering, 
      migration, and branching tubule formation of MDCK cells. To examine the role of 
      the Ras protein in the HGF/SF-induced responses, we constructed MDCK cell clones 
      expressing either inducible dominant-negative Ras or constitutively activated Ras 
      and analyzed their effects on responses of cells to HGF/SF. Induced expression of 
      dominant-negative Ras prevented cell dissociation required for cell scattering, 
      migration, and cystic formation as well as branching morphology required for 
      branching tubule formation. Constitutively activated Ras induced cell 
      dissociation, but not a scattered fibroblastic morphology even in the presence of 
      HGF/SF. MDCK cells expressing constitutively activated Ras migrated at a level 
      similar to that of wild-type MDCK cells stimulated by HGF/SF. MDCK cells 
      expressing constitutively activated Ras showed disorganized growth in 
      three-dimensional culture and did not form the branching tubule structures. These 
      results indicate that activation of the Ras protein is essential for the cell 
      scattering, migration, and branching tubule formation of MDCK cells induced by 
      HGF/SF, and a properly regulated activation is required for some stages of the 
      HGF/SF-induced responses of MDCK cells.
CI  - Copyright 2000 Academic Press.
FAU - Terauchi, R
AU  - Terauchi R
AD  - Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo 
      Institute of Technology, Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan.
FAU - Kitamura, N
AU  - Kitamura N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Cadherins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - Animals
MH  - Cadherins/metabolism
MH  - Cell Communication/physiology
MH  - Cell Line
MH  - Cell Movement/*physiology
MH  - Dogs
MH  - Epithelial Cells/*cytology/metabolism
MH  - Hepatocyte Growth Factor/pharmacology/*physiology
MH  - Immunoblotting
MH  - Membrane Proteins/metabolism
MH  - Microscopy, Fluorescence
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation
MH  - Transfection
MH  - Zonula Occludens-1 Protein
MH  - ras Proteins/genetics/metabolism/*physiology
EDAT- 2000/04/25 09:00
MHDA- 2000/05/20 09:00
CRDT- 2000/04/25 09:00
PHST- 2000/04/25 09:00 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 2000/04/25 09:00 [entrez]
AID - S0014-4827(00)94850-X [pii]
AID - 10.1006/excr.2000.4850 [doi]
PST - ppublish
SO  - Exp Cell Res. 2000 May 1;256(2):411-22. doi: 10.1006/excr.2000.4850.