PMID- 10772960
OWN - NLM
STAT- MEDLINE
DCOM- 20000522
LR  - 20181217
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 270
IP  - 3
DP  - 2000 Apr 21
TI  - Depolarizing stimuli reduce Ca(2+)/calmodulin-dependent protein kinase II 
      activity in islets of Langerhans.
PG  - 1119-23
AB  - Elevations in intracellular Ca(2+) ([Ca(2+)](i)) initiate insulin secretion from 
      pancreatic beta-cells, but the secretory responses become rapidly desensitised to 
      maintained elevations in [Ca(2+)](i). We have investigated the mechanisms 
      underlying the Ca(2+) desensitization of insulin secretion using electrically 
      permeabilized rat islets of Langerhans. Measurements of 
      Ca(2+)/calmodulin-dependent protein kinase II (CaMK II) enzyme activity and 
      immunoreactivity in permeabilized islets demonstrated Ca(2+)-induced reductions 
      in enzyme activity which could not be attributed to reductions in CaMK II 
      immunoreactive protein. Measurements in intact islets demonstrated that the 
      Ca(2+)-induced reduction of CaMK II activity was also operative in intact cells, 
      suggesting that this mechanism may have pathophysiological implications for 
      beta-cell function.
CI  - Copyright 2000 Academic Press.
FAU - Harris, T E
AU  - Harris TE
AD  - Reproduction and Development Group, Royal Veterinary College, Royal College 
      Street, London, NW1 0TU, United Kingdom.
FAU - Persaud, S J
AU  - Persaud SJ
FAU - Squires, P E
AU  - Squires PE
FAU - Jones, P M
AU  - Jones PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Insulin)
RN  - 660YQ98I10 (Potassium Chloride)
RN  - 982XCM1FOI (Tolbutamide)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - IY9XDZ35W2 (Glucose)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/pharmacology/*physiology
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Cell Membrane Permeability
MH  - Electrophysiology
MH  - Glucose/pharmacology
MH  - In Vitro Techniques
MH  - Insulin/*metabolism
MH  - Insulin Secretion
MH  - Islets of Langerhans/drug effects/enzymology/*physiology
MH  - Membrane Potentials
MH  - Potassium Chloride/pharmacology
MH  - Rats
MH  - Tolbutamide/pharmacology
EDAT- 2000/04/25 09:00
MHDA- 2000/06/08 09:00
CRDT- 2000/04/25 09:00
PHST- 2000/04/25 09:00 [pubmed]
PHST- 2000/06/08 09:00 [medline]
PHST- 2000/04/25 09:00 [entrez]
AID - S0006-291X(00)92563-4 [pii]
AID - 10.1006/bbrc.2000.2563 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Apr 21;270(3):1119-23. doi: 
      10.1006/bbrc.2000.2563.