PMID- 10773362
OWN - NLM
STAT- MEDLINE
DCOM- 20000619
LR  - 20220311
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 146
IP  - 1
DP  - 2000 Apr 20
TI  - A quantitative method for assessing the sensitizing potency of low molecular 
      weight chemicals using a local lymph node assay: employment of a regression 
      method that includes determination of the uncertainty margins.
PG  - 49-59
AB  - Risk assessment of sensitizing chemicals requires, besides hazard identification, 
      the assessment of potency. To examine the sensitizing capacity of low molecular 
      weight chemicals, a murine local lymph node assay (LLNA) was used. The 
      sensitizing capacity of known allergens was quantified by dose-response modeling. 
      At a stimulatory index (SI) of 3, the corresponding estimated concentration was 
      calculated (EC(3)), together with a confidence interval to take account of the 
      quality of the particular data set. We tested ten allergens 
      (ethyl-p-aminobenzoate (benzocaine), diethylamine (DEA), 2,4-dinitrochlorobenzene 
      (DNCB), 2-mercaptobenzothiazole (MBT), 4-ethoxymethylene 2-phenyl oxazol-5-one 
      (oxazolone), phthalic anhydride (PA), toluene diisocyanate (TDI), trimellitic 
      anhydride (TMA), tetramethylthiuramdisulfide (TMTD) and 
      zincdimethyldithiocarbamate (ZDMC)). Oxazolone showed the strongest sensitizing 
      potency followed in this order by DNCB, TDI, TMA, PA, TMTD, ZDMC, MBT, benzocaine 
      and DEA. The approach performed in this study is a way to accurately assess the 
      potency of sensitizing chemicals and thus a possibility for classification.
FAU - van Och, F M
AU  - van Och FM
AD  - National Institute of Public Health and the Environment (RIVM), P.O. Box 1, 3720 
      BA, Bilthoven, The Netherlands.h.van.loveren@rivm.nl
FAU - Slob, W
AU  - Slob W
FAU - de Jong, W H
AU  - de Jong WH
FAU - Vandebriel, R J
AU  - Vandebriel RJ
FAU - van Loveren, H
AU  - van Loveren H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Allergens)
RN  - 0 (Benzothiazoles)
RN  - 0 (Diethylamines)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Phthalic Anhydrides)
RN  - 0 (Thiazoles)
RN  - 0D771IS0FH (Thiram)
RN  - 15646-46-5 (Oxazolone)
RN  - 17X7AFZ1GH (Toluene 2,4-Diisocyanate)
RN  - 5RLR54Z22K (captax)
RN  - 73D8UA974J (Ziram)
RN  - 80T61EUU7H (trimellitic anhydride)
RN  - B035PIS86W (diethylamine)
RN  - U3RSY48JW5 (Benzocaine)
RN  - UVL263I5BJ (phthalic anhydride)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Benzocaine/immunology
MH  - Benzothiazoles
MH  - Dermatitis, Contact/*immunology
MH  - Diethylamines/immunology
MH  - Dinitrochlorobenzene/immunology
MH  - Dose-Response Relationship, Immunologic
MH  - Female
MH  - Linear Models
MH  - Lymph Nodes/*immunology/pathology
MH  - Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oxazolone/immunology
MH  - Phthalic Anhydrides/immunology
MH  - Regression Analysis
MH  - Scintillation Counting
MH  - Specific Pathogen-Free Organisms
MH  - Thiazoles/immunology
MH  - Thiram/immunology
MH  - Toluene 2,4-Diisocyanate/immunology
MH  - Ziram/immunology
EDAT- 2000/04/25 09:00
MHDA- 2000/06/24 11:00
CRDT- 2000/04/25 09:00
PHST- 2000/04/25 09:00 [pubmed]
PHST- 2000/06/24 11:00 [medline]
PHST- 2000/04/25 09:00 [entrez]
AID - S0300-483X(00)00165-7 [pii]
AID - 10.1016/s0300-483x(00)00165-7 [doi]
PST - ppublish
SO  - Toxicology. 2000 Apr 20;146(1):49-59. doi: 10.1016/s0300-483x(00)00165-7.