PMID- 10773683 OWN - NLM STAT- MEDLINE DCOM- 20000613 LR - 20171101 IS - 0301-0171 (Print) IS - 0301-0171 (Linking) VI - 88 IP - 1-2 DP - 2000 TI - FISH-microdissection (FISH-MD) analysis of complex chromosome rearrangements. PG - 114-8 AB - We combined the techniques of fluorescence in situ hybridization (FISH) and chromosomal microdissection in one experiment (FISH-MD). This novel method permits rapid identification of the composition, origin, and breakpoints of rearranged chromosomes. Rearranged chromosomes are first identified by multicolor-FISH, then the fluorophore-labeled derivative chromosomes are directly isolated by microdissection and reverse painted to identify the breakpoints. CI - Copyright 2000 S. Karger AG, Basel FAU - Weimer, J AU - Weimer J AD - Oncology Laboratory, Gynecology and Obstetrics Clinic, Christian-Albrechts University Kiel, Germany. Joerg.Weimer@t-online.de FAU - Kiechle, M AU - Kiechle M FAU - Arnold, N AU - Arnold N LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Switzerland TA - Cytogenet Cell Genet JT - Cytogenetics and cell genetics JID - 0367735 RN - 0 (Fluorescent Dyes) RN - 0 (Indoles) RN - 47165-04-8 (DAPI) SB - IM MH - Chromosome Aberrations/*genetics MH - Chromosome Banding/methods MH - Chromosome Breakage/genetics MH - Chromosome Painting/*methods MH - Color MH - Female MH - Fluorescent Dyes/metabolism MH - Humans MH - Indoles/metabolism MH - Karyotyping/methods MH - Light MH - Lymphocytes MH - Mixed Tumor, Mullerian/genetics MH - Mutagenesis, Insertional/genetics MH - Physical Chromosome Mapping/*methods MH - Polymerase Chain Reaction MH - Translocation, Genetic/genetics EDAT- 2000/04/25 09:00 MHDA- 2000/06/17 09:00 CRDT- 2000/04/25 09:00 PHST- 2000/04/25 09:00 [pubmed] PHST- 2000/06/17 09:00 [medline] PHST- 2000/04/25 09:00 [entrez] AID - 15502 [pii] AID - 10.1159/000015502 [doi] PST - ppublish SO - Cytogenet Cell Genet. 2000;88(1-2):114-8. doi: 10.1159/000015502.