PMID- 10774586
OWN - NLM
STAT- MEDLINE
DCOM- 20000731
LR  - 20190921
IS  - 0166-3542 (Print)
IS  - 0166-3542 (Linking)
VI  - 45
IP  - 1
DP  - 2000 Jan
TI  - Inhibitory effects of EICAR on infectious pancreatic necrosis virus replication.
PG  - 9-17
AB  - Recently, the antiviral 5-ethynyl-1-beta-D-ribofuranosylimidazole-4-carboxamide 
      (EICAR) was shown to inhibit the replication of the infectious pancreatic 
      necrosis virus (IPNV). In order to obtain more information about the mechanism of 
      the antiviral action of EICAR we studied its effect on viral macromolecules 
      synthesis. EICAR was found to inhibit IPNV messenger and genomic RNA synthesis. 
      To inhibit viral RNA synthesis, EICAR must be added at least 3 h before the start 
      of RNA synthesis. This suggests that EICAR does not directly affect the viral RNA 
      polymerization process. Moreover, the antiviral action of EICAR was reversed by 
      the exogenous addition of guanosine (5-50 microg/ml), but not adenosine or 
      cytidine (10-100 microg/ml). Our findings suggest that the antiviral action of 
      EICAR is mediated by a reduction of the intracellular guanosine 5'-triphosphate 
      (GTP) pool level, as has been observed with ribavirin and EICAR in other 
      biological systems.
FAU - Jashes, M
AU  - Jashes M
AD  - Departamento de Ciencias Biologicas, Facultad de Quimica y Biologia, Universidad 
      de Santiago de Chile.
FAU - Mlynarz, G
AU  - Mlynarz G
FAU - De Clercq, E
AU  - De Clercq E
FAU - Sandino, A M
AU  - Sandino AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Antiviral Res
JT  - Antiviral research
JID - 8109699
RN  - 0 (Antiviral Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Ribonucleosides)
RN  - 0 (Viral Proteins)
RN  - 118908-07-9 (5-ethynyl-1-ribofuranosylimidazole-4-carboxamide)
RN  - 12133JR80S (Guanosine)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/*pharmacology
MH  - Birnaviridae Infections/*virology
MH  - Cell Line
MH  - Guanosine/metabolism
MH  - Infectious pancreatic necrosis virus/*drug effects/physiology
MH  - RNA, Messenger/biosynthesis
MH  - RNA, Viral/biosynthesis
MH  - Ribonucleosides/*pharmacology
MH  - Salmon/embryology
MH  - Transcription, Genetic/drug effects
MH  - Viral Proteins/metabolism
MH  - Virus Replication/*drug effects
EDAT- 2000/04/25 09:00
MHDA- 2000/08/06 11:00
CRDT- 2000/04/25 09:00
PHST- 2000/04/25 09:00 [pubmed]
PHST- 2000/08/06 11:00 [medline]
PHST- 2000/04/25 09:00 [entrez]
AID - S0166-3542(99)00071-6 [pii]
AID - 10.1016/s0166-3542(99)00071-6 [doi]
PST - ppublish
SO  - Antiviral Res. 2000 Jan;45(1):9-17. doi: 10.1016/s0166-3542(99)00071-6.