PMID- 10775185
OWN - NLM
STAT- MEDLINE
DCOM- 20000807
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 62
IP  - 5
DP  - 2000 May
TI  - Intrafollicular concentrations of steroids and steroidogenic enzymes in relation 
      to follicular development in the mare.
PG  - 1335-43
AB  - The objective of the present study was to determine the changes in follicular 
      fluid steroid concentrations and in granulosa cell steroidogenic enzyme 
      expression during the follicular phase, in relation to follicular size and 
      physiological status in the mare. Follicular fluid and follicular cells were 
      recovered by ultrasound-guided follicular punctures either around the time of 
      emergence of the dominant follicle, at the end of the dominant follicle growth, 
      or at the preovulatory stage, after injection of gonadotropin to induce 
      ovulation. Cellular relative amounts of steroidogenic acute regulatory protein 
      (StAR), P450-side chain cleavage (P450(scc)), 3beta-hydroxysteroid dehydrogenase 
      (3betaHSD), 17alpha-hydroxylase, and aromatase were assessed by semiquantitative 
      Western blot and densitometry. Follicular fluid was assayed for cholesterol 
      concentrations by colorimetric assay and for progesterone, testosterone, and 
      estradiol-17beta concentrations by RIA. Intrafollicular concentrations of 
      progesterone and estradiol-17beta significantly increased in the dominant 
      follicle during growth. After injection of gonadotropin, follicular maturation 
      was characterized by a decrease in estradiol-17beta concentrations and a further 
      increase in progesterone concentrations. Granulosa cells from dominant follicles 
      had increased levels of StAR, P450(scc), 3betaHSD, and aromatase during growth, 
      but decreased levels during maturation. Levels of StAR, P450(scc), 3betaHSD, and 
      aromatase, as well as progesterone and estradiol-17beta, were lower in granulosa 
      cells from subordinate than from dominant follicles. We did not observe a 
      relationship between the steroidogenic activity of follicles and the capacity of 
      their enclosed oocytes to complete meiosis in vitro.
FAU - Belin, F
AU  - Belin F
AD  - INRA-Haras Nationaux, Reproduction Equine, P.R.M.D., 37380 Nouzilly, France.
FAU - Goudet, G
AU  - Goudet G
FAU - Duchamp, G
AU  - Duchamp G
FAU - Gerard, N
AU  - Gerard N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Enzymes)
RN  - 0 (Phosphoproteins)
RN  - 0 (Steroids)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 3XMK78S47O (Testosterone)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.14.14.1 (Aromatase)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/metabolism
MH  - Animals
MH  - Aromatase/metabolism
MH  - Cholesterol/metabolism
MH  - Cholesterol Side-Chain Cleavage Enzyme/metabolism
MH  - Enzymes/*metabolism
MH  - Estradiol/metabolism
MH  - Female
MH  - Follicular Fluid/metabolism
MH  - Granulosa Cells/metabolism
MH  - Horses
MH  - Ovarian Follicle/*metabolism/*physiology
MH  - Phosphoproteins/metabolism
MH  - Progesterone/blood/metabolism
MH  - Steroid 17-alpha-Hydroxylase/metabolism
MH  - Steroids/*metabolism
MH  - Testosterone/metabolism
EDAT- 2000/04/25 09:00
MHDA- 2000/08/12 11:00
CRDT- 2000/04/25 09:00
PHST- 2000/04/25 09:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/04/25 09:00 [entrez]
AID - 10.1095/biolreprod62.5.1335 [doi]
PST - ppublish
SO  - Biol Reprod. 2000 May;62(5):1335-43. doi: 10.1095/biolreprod62.5.1335.