PMID- 10775197
OWN - NLM
STAT- MEDLINE
DCOM- 20000807
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 62
IP  - 5
DP  - 2000 May
TI  - Expression and regulation of the CC-chemokine monocyte chemoattractant protein-1 
      in rat testicular cells in primary culture.
PG  - 1427-35
AB  - Testicular inflammation is classically observed in pathogenesis caused by 
      infectious agents, environmental toxins, trauma, or autoimmune reactions and can 
      lead to transitory or even permanent sterility. In these situations, a leukocyte 
      infiltration is generally encountered. Macrophage inflammatory proteins 
      (MIP)-1alpha and -1beta and monocyte chemoattractant protein-1 (MCP-1) are 
      CC-chemokines involved in macrophage and lymphocyte chemoattraction. In the 
      present study, using reverse transcription-polymerase chain reaction, Northern 
      blot, and a specific ELISA, we investigated whether or not these chemokines are 
      present within the testis and whether they are induced by a number of 
      proinflammatory cytokines and lipopolysaccharides (LPS). MIP-1alpha and MIP-1beta 
      were not detected in Sertoli cells, germ cells, peritubular cells, or Leydig 
      cells. In contrast, MCP-1 mRNA and protein were found to be expressed by control 
      isolated peritubular cells, and expression was markedly stimulated by 
      interleukin-1alpha and-1beta (IL-1alpha and IL-1beta), tumor necrosis factor 
      alpha (TNF-alpha), interferon gamma, and LPS. Leydig cells expressed MCP-1 when 
      stimulated by IL-1beta. In contrast, MCP-1 was not found to be produced by 
      Sertoli cells or germ cells as established by Northern blot and ELISA techniques. 
      The kinetics of MCP-1 production by peritubular cells, as demonstrated by 
      expression as early as 8 h poststimulation, are compatible with there being a 
      rapid mobilization of these cells and this chemokine in an inflammatory process. 
      Moreover, MCP-1 production by peritubular cells after half-maximal stimulation by 
      LPS, TNF-alpha, and IL-1beta (2 pg/ml-0.9 ng/ml) is also compatible with the 
      physiologic concentrations of the proinflammatory cytokines generally found in an 
      inflammatory site. It is concluded that MCP-1 is produced by Leydig cells and 
      peritubular cells and that it could be involved in the mobilization and migration 
      of leukocytes observed during testicular inflammation.
FAU - Aubry, F
AU  - Aubry F
AD  - GERM-INSERM U. 435, Universite de Rennes I, Campus de Beaulieu, 35042 Rennes 
      cedex, Bretagne, France.
FAU - Habasque, C
AU  - Habasque C
FAU - Satie, A P
AU  - Satie AP
FAU - Jegou, B
AU  - Jegou B
FAU - Samson, M
AU  - Samson M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics/*metabolism
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Gene Expression Regulation
MH  - Interleukin-1/pharmacology
MH  - Leydig Cells/physiology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophage Inflammatory Proteins/*genetics/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sertoli Cells/physiology
MH  - Spermatozoa/physiology
MH  - Testis/cytology/drug effects/*metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2000/04/25 09:00
MHDA- 2000/08/12 11:00
CRDT- 2000/04/25 09:00
PHST- 2000/04/25 09:00 [pubmed]
PHST- 2000/08/12 11:00 [medline]
PHST- 2000/04/25 09:00 [entrez]
AID - 10.1095/biolreprod62.5.1427 [doi]
PST - ppublish
SO  - Biol Reprod. 2000 May;62(5):1427-35. doi: 10.1095/biolreprod62.5.1427.