PMID- 10777147
OWN - NLM
STAT- MEDLINE
DCOM- 20000607
LR  - 20061115
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 16
IP  - 6
DP  - 2000 Apr 10
TI  - Molecular characterization of feline interleukin 16: chemotactic activity and 
      effect on feline immunodeficiency virus infection and/or replication.
PG  - 569-75
AB  - Interleukin 16 (IL-16) has been shown to diminish HIV and SIV replication through 
      inhibition of HIV and SIV mRNA transcription. To evaluate its role in the FIV cat 
      model, we cloned and expressed feline IL-16 and determined its ability to induce 
      chemotaxis as well as to inhibit FIV replication in cultured PBMCs. Sequence 
      comparison of rfIL-16 with human, African green monkey, rhesus macaque, and mouse 
      IL-16 showed 84.2, 84.5, 84.4, and 79.4% identity at the nucleotide sequence 
      level and 93, 91.5, 90.7, and 87.2% identity at the amino acid sequence level, 
      respectively. Biocharacterization of rfIL-16 revealed potent induction of 
      chemotaxis (p < 0.05). In addition, p24 production from feline PBMCs infected 
      with FIV Zurich 2 in vitro was decreased up to 87% (p < 0.05). These data 
      demonstrate biologic and antiviral functionality of rfIL-16.
FAU - Leutenegger, C M
AU  - Leutenegger CM
AD  - Department of Internal Veterinary Medicine, University of Zurich, Switzerland. 
      cmleutenegger@ucdavis.edu
FAU - Huder, J B
AU  - Huder JB
FAU - Mislin, C N
AU  - Mislin CN
FAU - Lahrtz, F
AU  - Lahrtz F
FAU - Hofmann-Lehmann, R
AU  - Hofmann-Lehmann R
FAU - Pedersen, N C
AU  - Pedersen NC
FAU - Lutz, H
AU  - Lutz H
LA  - eng
SI  - GENBANK/AF003701
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (Interleukin-16)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cats
MH  - Cells, Cultured
MH  - Chemotaxis, Leukocyte/*drug effects
MH  - Cloning, Molecular
MH  - Escherichia coli/genetics
MH  - Immunodeficiency Virus, Feline/*drug effects
MH  - Interleukin-16/biosynthesis/*genetics/*pharmacology
MH  - Leukocytes, Mononuclear/*drug effects/physiology/virology
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/pharmacology
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Specific Pathogen-Free Organisms
MH  - Virus Replication/drug effects
EDAT- 2000/04/25 09:00
MHDA- 2000/06/10 09:00
CRDT- 2000/04/25 09:00
PHST- 2000/04/25 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/04/25 09:00 [entrez]
AID - 10.1089/088922200308981 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 2000 Apr 10;16(6):569-75. doi: 
      10.1089/088922200308981.