PMID- 10777703
OWN - NLM
STAT- MEDLINE
DCOM- 20000612
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 271
IP  - 1
DP  - 2000 Apr 29
TI  - Mechanism of regulation of HGF/SF gene expression in fibroblasts by TGF-beta1.
PG  - 203-11
AB  - The effect of transforming growth factor beta 1 (TGF-beta1) on levels of 
      hepatocyte growth factor/scatter factor (HGF/SF) gene transcripts was 
      investigated in the human lung embryonic fibroblast cell line, MRC-5. TGF-beta1 
      markedly reduced the expression of the 6. 0-kb and 3.0-kb HGF/SF mRNA, which 
      encode full-length HGF/SF, but it had little effect on the expression of the 
      alternatively spliced 1. 5-kb mRNA, which encodes NK2, a competitive HGF/SF 
      antagonist. Using actinomycin D to block RNA synthesis, it was observed that 
      TGF-beta1 had little effect on the stability of the 1.5-kb NK2 mRNA but increased 
      the rate of degradation of the 6.0- and 3.0-kb HGF/SF mRNA transcripts by a 
      mechanism that was dependent on new protein synthesis. TGF-beta1 minimally 
      increased rather than reduced HGF/SF promoter activity in cells transiently 
      transfected with chloramphenicol acetyltransferase (CAT) reporter genes driven by 
      HGF/SF gene 5'-flanking sequences. In MRC-5 cells, TGF-beta1 modulates HGF/SF 
      gene transcripts at the posttranscriptional level in order to favour expression 
      of the 1.5-kb mRNA that encodes the truncated protein NK2.
CI  - Copyright 2000 Academic Press.
FAU - Harrison, P
AU  - Harrison P
AD  - Institute of Liver Studies, GKT School of Medicine, King's Denmark Hill Campus, 
      Bessemer Road, London, SE5 9PJ, United Kingdom.
FAU - Bradley, L
AU  - Bradley L
FAU - Bomford, A
AU  - Bomford A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 3.1.- (Ribonucleases)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Blotting, Northern
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cycloheximide/pharmacology
MH  - Dactinomycin/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Fibroblasts/*metabolism
MH  - *Gene Expression Regulation, Developmental
MH  - Hepatocyte Growth Factor/genetics/*metabolism
MH  - Humans
MH  - Promoter Regions, Genetic
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Recombinant Proteins/metabolism
MH  - Ribonucleases/metabolism
MH  - Time Factors
MH  - Transforming Growth Factor beta/*pharmacology
MH  - beta-Galactosidase/metabolism
EDAT- 2000/04/25 09:00
MHDA- 2000/06/17 09:00
CRDT- 2000/04/25 09:00
PHST- 2000/04/25 09:00 [pubmed]
PHST- 2000/06/17 09:00 [medline]
PHST- 2000/04/25 09:00 [entrez]
AID - S0006-291X(00)92612-3 [pii]
AID - 10.1006/bbrc.2000.2612 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Apr 29;271(1):203-11. doi: 
      10.1006/bbrc.2000.2612.