PMID- 10779683
OWN - NLM
STAT- MEDLINE
DCOM- 20020226
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1474
IP  - 3
DP  - 2000 May 1
TI  - NADH-dependent decavanadate reductase, an alternative activity of NADP-specific 
      isocitrate dehydrogenase protein.
PG  - 321-30
AB  - The well known NADP-specific isocitrate dehydrogenase (IDH) obtained from pig 
      heart was found to oxidize NADH with accompanying consumption of oxygen 
      (NADH:O(2)=1:1) in presence of polyvanadate. This activity of the soluble 
      IDH-protein has the following features common with the previously described 
      membrane-enzymes: heat-sensitive, active only with NADH but not NADPH, increased 
      rates in acidic pH, dependence on concentrations of the enzyme, NADH, 
      decavanadate and metavanadate (the two constituents of polyvanadate), and 
      sensitivity to SOD and EDTA. Utilizing NADH as the electron source the IDH 
      protein was able to reduce decavanadate but not metavanadate. This reduced form 
      of vanadyl (V(IV)) was similar in its eight-band electron spin resonance spectrum 
      to vanadyl sulfate but had a 20-fold higher absorbance at its 700 nm peak. This 
      decavanadate reductase activity of the protein was sensitive to heat and was not 
      inhibited by SOD and EDTA. The IDH protein has the additional enzymic activity of 
      NADH-dependent decavanadate reductase and is an example of "one protein--many 
      functions".
FAU - Rao, A V
AU  - Rao AV
AD  - Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
FAU - Ramasarma, T
AU  - Ramasarma T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Buffers)
RN  - 0U46U6E8UK (NAD)
RN  - 3WHH0066W5 (Vanadates)
RN  - EC 1.1.1.41 (Isocitrate Dehydrogenase)
RN  - EC 1.6.- (NADH, NADPH Oxidoreductases)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Ascorbic Acid/chemistry
MH  - Binding Sites
MH  - Buffers
MH  - Catalysis
MH  - Electron Spin Resonance Spectroscopy
MH  - Hydrogen-Ion Concentration
MH  - Isocitrate Dehydrogenase/*chemistry
MH  - NAD/chemistry
MH  - NADH, NADPH Oxidoreductases/*chemistry
MH  - Oxygen Consumption
MH  - Vanadates/*chemistry
EDAT- 2000/04/26 00:00
MHDA- 2002/02/28 10:01
CRDT- 2000/04/26 00:00
PHST- 2000/04/26 00:00 [pubmed]
PHST- 2002/02/28 10:01 [medline]
PHST- 2000/04/26 00:00 [entrez]
AID - S0304-4165(00)00026-X [pii]
AID - 10.1016/s0304-4165(00)00026-x [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2000 May 1;1474(3):321-30. doi: 
      10.1016/s0304-4165(00)00026-x.