PMID- 10779802
OWN - NLM
STAT- MEDLINE
DCOM- 20000522
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 164
IP  - 9
DP  - 2000 May 1
TI  - IFN-gamma and TNF regulate macrophage expression of the chemotactic S100 protein 
      S100A8.
PG  - 4916-23
AB  - The murine calcium-binding protein S100A8 is a potent chemoattractant for 
      neutrophils and monocytes in vivo and in vitro but may also play a protective 
      role. We show that the kinetics of induction of S100A8 mRNA in elicited murine 
      macrophages (Mac) by LPS, IFN-gamma, and TNF were distinct from the C-C 
      chemokines monocyte chemoattractant protein-1 (MCP-1), macrophage-inflammatory 
      protein-1alpha (MIP-1alpha), and RANTES. Monomeric S100A8 was predominantly 
      secreted. IFN substantially increased S100A8 mRNA levels after 1 h with optimal 
      induction after 12 h; induction by TNF was slower and more sustained. TNF did not 
      up-regulate MCP-1 and MIP-1alpha mRNA in these cells. Luciferase reporter assays 
      confirmed that LPS and IFN induce S100A8 gene transcription and mRNA in 
      LPS-treated Mac showed little decay over 16 h, whereas transcripts induced by IFN 
      and TNF were markedly less stable. Newly synthesized proteins may be required for 
      mRNA transcription and stabilization in response to LPS. S100A9 associates with 
      A8 in neutrophils, but was not coinduced with S100A8. S100A8 gene induction in 
      Mac stimulated with LPS and IFN may be modulated by mobilization of intracellular 
      Ca2+ concentration from distinct intracellular stores and/or the extracellular 
      compartment and by distinct pathways involving protein kinase C and leading to 
      activation of mitogen-activated protein kinase.
FAU - Xu, K
AU  - Xu K
AD  - Cytokine Research Unit, School of Pathology, Faculty of Medicine, University of 
      New South Wales, Sydney, Australia.
FAU - Geczy, C L
AU  - Geczy CL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Calgranulin A)
RN  - 0 (Calgranulin B)
RN  - 0 (Chemokines, CC)
RN  - 0 (Chemotactic Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (S100 Proteins)
RN  - 0 (S100a8 protein, mouse)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation/biosynthesis
MH  - Calcium-Binding Proteins/biosynthesis
MH  - Calgranulin A
MH  - Calgranulin B
MH  - Chemokines, CC/genetics/physiology
MH  - Chemotactic Factors/*biosynthesis/genetics
MH  - Chemotaxis, Leukocyte/immunology
MH  - Gene Expression Regulation/immunology
MH  - Interferon-gamma/*physiology
MH  - Macrophage Activation
MH  - Macrophages, Peritoneal/immunology/*metabolism
MH  - Mice
MH  - RNA, Messenger/biosynthesis
MH  - *S100 Proteins
MH  - Transcriptional Activation
MH  - Tumor Necrosis Factor-alpha/*physiology
MH  - Up-Regulation/immunology
EDAT- 2000/04/26 09:00
MHDA- 2000/06/08 09:00
CRDT- 2000/04/26 09:00
PHST- 2000/04/26 09:00 [pubmed]
PHST- 2000/06/08 09:00 [medline]
PHST- 2000/04/26 09:00 [entrez]
AID - ji_v164n9p4916 [pii]
AID - 10.4049/jimmunol.164.9.4916 [doi]
PST - ppublish
SO  - J Immunol. 2000 May 1;164(9):4916-23. doi: 10.4049/jimmunol.164.9.4916.