PMID- 10780169
OWN - NLM
STAT- MEDLINE
DCOM- 20000516
LR  - 20200429
IS  - 0931-1793 (Print)
IS  - 1439-0450 (Electronic)
IS  - 0931-1793 (Linking)
VI  - 47
IP  - 1
DP  - 2000 Feb
TI  - Porcine reproductive and respiratory syndrome virus (PRRSV): kinetics of 
      infection in lymphatic organs and lung.
PG  - 9-25
AB  - Pigs were infected by the oronasal route with European isolates of the porcine 
      reproductive and respiratory syndrome virus (PRRSV; I10 and Cobbelsdorf). The 
      kinetics of infection in lymphatic organs and the lung were analysed by 
      immunofluorescence detection of virus antigen, re-isolation of the virus and 
      reverse transcription--polymerase chain reaction (RT-PCR) for PRRSV-specific RNA. 
      The kinetics of PRRSV infection proceeded in three phases, irrespective of the 
      varying infestation of lymphatic organs within the first days post-infection 
      (p.i.). First, an early acute infection of lymphatic organs developed within the 
      first week and was characterized by a high number of antigen-positive 
      macrophages. Second, a delayed acute infection of the lung was observed, which 
      was most pronounced during the second and third week p.i. when a high number of 
      infected alveolar macrophages was observed. The acute infection of lymphatic 
      organs had resolved at this time. Infected cells in the lung were predominantly 
      located in pneumonic lesions. Third, a persistent infection was demonstrated by 
      RT-PCR and immunohistology when the experiments were terminated at day 49 p.i. 
      The virus persisted in lymphatic organs, especially in the tonsils, and in the 
      lung. At this stage, indications for a re-occurrence of acute infection were 
      observed in restricted areas of the lung.
FAU - Beyer, J
AU  - Beyer J
AD  - Federal Research Centre for Virus Diseases of Animals, 
      Friedrich-Loeffler-Institutes, Insel Riems, Germany.
FAU - Fichtner, D
AU  - Fichtner D
FAU - Schirrmeier, H
AU  - Schirrmeier H
FAU - Polster, U
AU  - Polster U
FAU - Weiland, E
AU  - Weiland E
FAU - Wege, H
AU  - Wege H
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - J Vet Med B Infect Dis Vet Public Health
JT  - Journal of veterinary medicine. B, Infectious diseases and veterinary public 
      health
JID - 100955260
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Animals, Suckling
MH  - Antigens, Viral/analysis
MH  - DNA Primers/chemistry
MH  - DNA, Viral/chemistry
MH  - Electrophoresis, Agar Gel/veterinary
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect/veterinary
MH  - Kinetics
MH  - Lung/pathology/*virology
MH  - Lymphatic System/pathology/*virology
MH  - Pneumonia, Viral/immunology/*veterinary/virology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/immunology/isolation 
      & purification/*physiology
MH  - Pregnancy
MH  - RNA, Viral/chemistry/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
PMC - PMC7183809
EDAT- 2000/04/26 09:00
MHDA- 2000/06/03 09:00
PMCR- 2020/04/26
CRDT- 2000/04/26 09:00
PHST- 2000/04/26 09:00 [pubmed]
PHST- 2000/06/03 09:00 [medline]
PHST- 2000/04/26 09:00 [entrez]
PHST- 2020/04/26 00:00 [pmc-release]
AID - ZPH305 [pii]
AID - 10.1046/j.1439-0450.2000.00305.x [doi]
PST - ppublish
SO  - J Vet Med B Infect Dis Vet Public Health. 2000 Feb;47(1):9-25. doi: 
      10.1046/j.1439-0450.2000.00305.x.