PMID- 10780676
OWN - NLM
STAT- MEDLINE
DCOM- 20000509
LR  - 20230216
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 80
IP  - 4
DP  - 2000 Apr
TI  - Human dendritic cells in the severe combined immunodeficiency mouse model: their 
      potentiating role in the allergic reaction.
PG  - 605-14
AB  - Dendritic cells (DCs) are present in the lungs and airways of healthy and 
      allergic subjects where they are exposed to inhaled antigens. After the uptake of 
      antigens, DCs migrate to lymphoid organs where T cells initiate and control the 
      immune response. The migratory properties of DCs are an essential component of 
      their function but remain unclear in the situation of allergic diseases. To 
      better understand the role of DCs in response to allergens, we first investigated 
      their presence in an original experimental model of allergic asthma: the 
      humanized severe combined immunodeficiency (SCID) mouse reconstituted with 
      peripheral blood mononuclear cells from patients sensitive to Dermatophagoides 
      pteronyssinus (Dpt). Human DCs were detected in lungs of mice developing an 
      inflammatory pulmonary infiltrate and appeared to be mainly located in the 
      alveolar spaces. In a second step, human DCs were generated in vitro from 
      monocytes and injected into naive SCID mice exposed or not exposed to Dpt 
      aerosols. Their migratory behavior was explored, as well as their potential role 
      in modulating the IgE production after exposure to Dpt. After exposure to Dpt, 
      the number of DCs present in airways decreased, while it increased into the 
      spleen and thymus of the mice. The IgE production increased in the presence of 
      DCs as compared with mice not injected with DCs. These results suggest that DCs 
      may play a role in the pulmonary allergic reaction developed in response to Dpt 
      in SCID mice.
FAU - Hammad, H
AU  - Hammad H
AD  - Unite INSERM U-416, Institut Pasteur de Lille, France.
FAU - Duez, C
AU  - Duez C
FAU - Fahy, O
AU  - Fahy O
FAU - Tsicopoulos, A
AU  - Tsicopoulos A
FAU - Andre, C
AU  - Andre C
FAU - Wallaert, B
AU  - Wallaert B
FAU - Lebecque, S
AU  - Lebecque S
FAU - Tonnel, A B
AU  - Tonnel AB
FAU - Pestel, J
AU  - Pestel J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Glycoproteins)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigens, Dermatophagoides
MH  - Dendritic Cells/*immunology/transplantation
MH  - Glycoproteins/*immunology
MH  - Humans
MH  - Hypersensitivity/*immunology
MH  - Mice
MH  - Mice, SCID
MH  - Severe Combined Immunodeficiency/*immunology
EDAT- 2000/04/26 09:00
MHDA- 2000/05/16 09:00
CRDT- 2000/04/26 09:00
PHST- 2000/04/26 09:00 [pubmed]
PHST- 2000/05/16 09:00 [medline]
PHST- 2000/04/26 09:00 [entrez]
AID - S0023-6837(22)02195-X [pii]
AID - 10.1038/labinvest.3780065 [doi]
PST - ppublish
SO  - Lab Invest. 2000 Apr;80(4):605-14. doi: 10.1038/labinvest.3780065.