PMID- 10782886
OWN - NLM
STAT- MEDLINE
DCOM- 20000509
LR  - 20190921
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 436
IP  - 3
DP  - 2000 Mar
TI  - Feasibility of simultaneous fluorescence immunophenotyping and fluorescence in 
      situ hybridization study for the detection of estrogen receptor expression and 
      deletions of the estrogen receptor gene in breast carcinoma cell lines.
PG  - 271-5
AB  - For the first time, combined immunophenotyping and fluorescence in situ 
      hybridization (FISH) technique according to the "fluorescence immunophenotyping 
      and interphase cytogenetics as a tool for investigation of neoplasms" (FICTION) 
      technique have been successfully applied in solid tumors. Thus, we were able to 
      visualize the antigen expression of cells with chromosomal deletions of a tumor 
      suppressor region directly. In six breast carcinoma cell lines, we investigated 
      the correlation between estrogen receptor (ER) expression status and deletions of 
      the estrogen receptor gene (ESR). To screen for deletions of the ESR gene, 
      dual-color FISH was performed with a YAC (yeast artificial chromosome) probe 
      containing the ESR gene and, as internal control, with a centromeric probe of 
      chromosome 6. Deletions of the ESR gene were detected in four of six cell lines. 
      For direct comparison of ER expression with the copy number of the ESR gene at 
      the single cell level, immunophenotyping with mouse anti-human ER antibody was 
      combined with FISH with the YAC probe containing the ESR gene according to the 
      FICTION technique. There was no correlation between lack of or reduced ER 
      expression and deletions of the ESR gene. One cell line with deletions of the ESR 
      gene did express ER on the protein level, while another cell line without a 
      deletion did not. Cells with deletions of the ESR gene were either ER expression 
      positive or negative. The staining intensity of ER expression was not associated 
      with the copy number of the ESR gene. Thus, this FICTION study unequivocally 
      shows that deletions of the ESR gene are not the major cause of absent or reduced 
      ER expression in breast carcinoma cell lines.
FAU - Zhang, Y
AU  - Zhang Y
AD  - Department of Human Genetics, University of Kiel, Germany.
FAU - Siebert, R
AU  - Siebert R
FAU - Matthiesen, P
AU  - Matthiesen P
FAU - Harder, S
AU  - Harder S
FAU - Theile, M
AU  - Theile M
FAU - Scherneck, S
AU  - Scherneck S
FAU - Schlegelberger, B
AU  - Schlegelberger B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Receptors, Estrogen)
SB  - IM
MH  - Animals
MH  - Breast Neoplasms/*genetics/*metabolism
MH  - Female
MH  - Gene Deletion
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Receptors, Estrogen/*biosynthesis/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2000/04/27 09:00
MHDA- 2000/05/16 09:00
CRDT- 2000/04/27 09:00
PHST- 2000/04/27 09:00 [pubmed]
PHST- 2000/05/16 09:00 [medline]
PHST- 2000/04/27 09:00 [entrez]
AID - 10.1007/s004280050040 [doi]
PST - ppublish
SO  - Virchows Arch. 2000 Mar;436(3):271-5. doi: 10.1007/s004280050040.