PMID- 10783401
OWN - NLM
STAT- MEDLINE
DCOM- 20000602
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 20
IP  - 10
DP  - 2000 May 15
TI  - Cervical dorsal rhizotomy increases brain-derived neurotrophic factor and 
      neurotrophin-3 expression in the ventral spinal cord.
PG  - RC77
AB  - Although neurotrophic factors have been implicated in several forms of 
      neuroplasticity, little is known concerning their potential role in spinal 
      plasticity. Cervical dorsal rhizotomy (CDR) enhances serotonin terminal density 
      near (spinal) phrenic motoneurons and serotonin-dependent long-term facilitation 
      of phrenic motor output (Kinkead et al., 1998). We tested the hypothesis that 
      selected neurotrophic factors change in a manner consistent with an involvement 
      in this model of spinal plasticity. Brain-derived neurotrophic factor (BDNF), 
      neurotrophin-3 (NT-3), glial cell line-derived neurotrophic factor (GDNF), and 
      transforming growth factor-beta(1) (TGF-beta(1)) concentrations were measured 
      (ELISA) in three regions of interest to respiratory control: (1) ventral cervical 
      spinal segments associated with the phrenic motor nucleus (C3-C6), (2) ventral 
      thoracic spinal segments associated with inspiratory intercostal motor output 
      (T3-T6) and (3) the diaphragm. Tissues were harvested from rats 7 d after 
      bilateral CDR and compared with sham-operated and unoperated control rats. CDR 
      increased BDNF (110%; p = 0.002) and NT-3 (100%; p = 0.002) in the cervical and 
      NT-3 in the thoracic spinal cord (98%; p = 0.009). GDNF and TGF-beta(1) were not 
      altered by CDR in any tissue. Immunohistochemistry localized BDNF and NT-3 to 
      motoneurons and interneurons of the ventral spinal cord. These studies provide 
      novel, suggestive evidence that BDNF and NT-3, possibly through their trophic 
      effects on serotonergic neurons and/or motoneurons, may underlie 
      serotonin-dependent plasticity in (spinal) respiratory motor control after CDR.
FAU - Johnson, R A
AU  - Johnson RA
AD  - Department of Comparative Biosciences, School of Veterinary Medicine, University 
      of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
FAU - Okragly, A J
AU  - Okragly AJ
FAU - Haak-Frendscho, M
AU  - Haak-Frendscho M
FAU - Mitchell, G S
AU  - Mitchell GS
LA  - eng
GR  - HL03874/HL/NHLBI NIH HHS/United States
GR  - HL36780/HL/NHLBI NIH HHS/United States
GR  - HL53319/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Diaphragm/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Interneurons/metabolism
MH  - Male
MH  - Motor Neurons/metabolism
MH  - *Nerve Growth Factors
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurotrophin 3/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rhizotomy
MH  - Spinal Cord/*metabolism/physiology
MH  - Transforming Growth Factor beta/metabolism
PMC - PMC6772689
EDAT- 2000/04/28 00:00
MHDA- 2000/06/10 00:00
PMCR- 2000/11/15
CRDT- 2000/04/28 00:00
PHST- 2000/04/28 00:00 [pubmed]
PHST- 2000/06/10 00:00 [medline]
PHST- 2000/04/28 00:00 [entrez]
PHST- 2000/11/15 00:00 [pmc-release]
AID - 20004209 [pii]
AID - 4209 [pii]
AID - 10.1523/JNEUROSCI.20-10-j0005.2000 [doi]
PST - ppublish
SO  - J Neurosci. 2000 May 15;20(10):RC77. doi: 10.1523/JNEUROSCI.20-10-j0005.2000.