PMID- 10783905
OWN - NLM
STAT- MEDLINE
DCOM- 20000518
LR  - 20161020
IS  - 0862-8408 (Print)
IS  - 0862-8408 (Linking)
VI  - 48
IP  - 6
DP  - 1999
TI  - Differences between cation-osmotic hemolysis and filterability in exaprolol- and 
      glutaraldehyde-treated human red blood cells.
PG  - 411-6
AB  - The changes in human red blood cell microrheology in different glutaraldehyde 
      (3.0 and 5.0 x 10(-6) mol x l(-1)) and exaprolol (2.5 and 5.0 x 10(-4) mol x 
      l(-1)) concentrations were studied. The method of millipore filtration was 
      compared with the method of cation-osmotic hemolysis. Both drugs prolonged the 
      filtration time. Cation-osmotic hemolysis in glutaraldehyde-treated cells was 
      significantly lower in comparison with the control group. On the other hand, 
      there was a significant increase in cation-osmotic hemolysis in exaprolol-treated 
      cells. Besides cation-osmotic hemolysis and filterability of erythrocytes, we 
      evaluated the medium cell volume (MCV) and the medium cell hemoglobin 
      concentration (MCHC). No changes in MCV and MCHC in glutaraldehyde-treated cells 
      were observed. However, the MCV was significantly lower and the MCHC was 
      significantly higher in exaprolol-treated cells. In conclusion, we suggest that 
      the method of cation-osmotic hemolysis is more sensitive than the filtration 
      method for determination of red blood cell microrheology.
FAU - Mojzis, J
AU  - Mojzis J
AD  - Department of Pharmacology, Medical Faculty, Safarik University, Kosice, Slovak 
      Republic. mojzis@central.medic.upjs.sk
FAU - Nicak, A
AU  - Nicak A
FAU - Linkova, A
AU  - Linkova A
FAU - Jandosekova, M
AU  - Jandosekova M
FAU - Mirossay, L
AU  - Mirossay L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
RN  - 0 (Adrenergic beta-Antagonists)
RN  - 0 (Cations)
RN  - 0 (Disinfectants)
RN  - 0 (Propanolamines)
RN  - Q4XX54I93R (exaprolol)
RN  - T3C89M417N (Glutaral)
SB  - IM
MH  - Adrenergic beta-Antagonists/*pharmacology
MH  - Adult
MH  - Cations/pharmacology
MH  - Disinfectants/*pharmacology
MH  - Erythrocyte Deformability/*drug effects/physiology
MH  - Glutaral/*pharmacology
MH  - Hemolysis/*drug effects/physiology
MH  - Humans
MH  - Middle Aged
MH  - Osmotic Pressure
MH  - Propanolamines/*pharmacology
EDAT- 2000/04/28 09:00
MHDA- 2000/05/20 09:00
CRDT- 2000/04/28 09:00
PHST- 2000/04/28 09:00 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 2000/04/28 09:00 [entrez]
PST - ppublish
SO  - Physiol Res. 1999;48(6):411-6.