PMID- 10787051
OWN - NLM
STAT- MEDLINE
DCOM- 20000727
LR  - 20240109
IS  - 0032-0935 (Print)
IS  - 0032-0935 (Linking)
VI  - 210
IP  - 4
DP  - 2000 Mar
TI  - Inhibitors of the carrier-mediated influx of auxin in suspension-cultured tobacco 
      cells.
PG  - 580-8
AB  - Active auxin transport in plant cells is catalyzed by two carriers working in 
      opposite directions at the plasma membrane, the influx and efflux carriers. A 
      role for the efflux carrier in polar auxin transport (PAT) in plants has been 
      shown from studies using phytotropins. Phytotropins have been invaluable in 
      demonstrating that PAT is essential to ensure polarized and coordinated growth 
      and to provide plants with the capacity to respond to environmental stimuli. 
      However, the function of the influx carrier at the whole-plant level is unknown. 
      Our work aims to identify new auxin-transport inhibitors which could be employed 
      to investigate its function. Thirty-five aryl and aryloxyalkylcarboxylic acids 
      were assayed for their ability to perturb the accumulation of 
      2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene-1-acetic acid (1-NAA) in 
      suspension-cultured tobacco (Nicotiana tabacum L.) cells. As 2,4-D and 1-NAA are 
      preferentially transported by the influx and efflux carriers, respectively, 
      accumulation experiments utilizing synthetic auxins provide independant 
      information on the activities of both carriers. The majority (60%) of compounds 
      half-inhibited the carrier-mediated influx of [14C]2,4-D at concentrations of 
      less than 10 microM. Most failed to interfere with [3H]NAA efflux, at least in 
      the short term. Even though they increasingly perturbed auxin efflux when given a 
      prolonged treatment, several compounds were much better at discriminating between 
      influx and efflux carrier activities than naphthalene-2-acetic acid which is 
      commonly employed to investigate influx-carrier properties. Structure-activity 
      relationships and factors influencing ligand specificity with regard to auxin 
      carriers are discussed.
FAU - Imhoff, V
AU  - Imhoff V
AD  - Institut des Sciences Vegetales, UPR 0040, CNRS, Gif-sur-Yvette, France.
FAU - Muller, P
AU  - Muller P
FAU - Guern, J
AU  - Guern J
FAU - Delbarre, A
AU  - Delbarre A
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
RN  - 0 (Indoleacetic Acids)
RN  - 0 (Naphthaleneacetic Acids)
RN  - 0 (Phenoxyacetates)
RN  - 0 (Phenylacetates)
RN  - 0 (Pyrenes)
RN  - 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
RN  - 33T7G7757C (1-naphthaleneacetic acid)
SB  - IM
MH  - 2,4-Dichlorophenoxyacetic Acid/antagonists & inhibitors/chemistry/*metabolism
MH  - Biological Transport
MH  - Cells, Cultured
MH  - Indoleacetic Acids/antagonists & inhibitors/chemistry/*metabolism
MH  - Naphthaleneacetic Acids/antagonists & inhibitors/chemistry/*metabolism
MH  - Phenoxyacetates/chemistry/pharmacology
MH  - Phenylacetates/chemistry/pharmacology
MH  - *Plants, Toxic
MH  - Pyrenes/chemistry/pharmacology
MH  - Structure-Activity Relationship
MH  - Nicotiana/chemistry/*metabolism
EDAT- 2000/04/29 09:00
MHDA- 2000/08/01 11:00
CRDT- 2000/04/29 09:00
PHST- 2000/04/29 09:00 [pubmed]
PHST- 2000/08/01 11:00 [medline]
PHST- 2000/04/29 09:00 [entrez]
AID - 10.1007/s004250050047 [pii]
AID - 10.1007/s004250050047 [doi]
PST - ppublish
SO  - Planta. 2000 Mar;210(4):580-8. doi: 10.1007/s004250050047.