PMID- 10787100
OWN - NLM
STAT- MEDLINE
DCOM- 20000606
LR  - 20190905
IS  - 0090-4341 (Print)
IS  - 0090-4341 (Linking)
VI  - 38
IP  - 4
DP  - 2000 May
TI  - Ecotoxicological assessment of persistent organic and heavy metal contamination 
      in Hong Kong coastal sediment.
PG  - 486-93
AB  - The aim of the present project is to determine the feasibility of measuring 
      hepatic cytochrome P4501A1 (CYP1A1) and metallothionein (MT) mRNA in fish as an 
      integrative measurement of persistent organic pollutants (POPs) and heavy metal 
      contamination in sediment arising in Hong Kong. Sediment samples were collected 
      from different sites, including Victoria Harbour (VS6), Yim Tin Tsai (YTT) at 
      Tolo Harbour, Mai Po marshes (MPM) at Deep Bay, and Southern Waters (SS6) of 
      coastal waters. The samples were analyzed for total and extractable 
      concentrations of Cd, Cu, Ni, Zn, and Pb, as well as PCBs and PAHs. In addition, 
      biomarker responses were studied in tilapia exposed experimentally to coastal 
      sediment for 7 days. Using RT-PCR technique, hepatic CYP1A1 and MT mRNA were 
      measured. Three control groups were used, including one negative control group 
      maintained in sea water only; the second and third positive control groups were 
      in sea water but were intraperitoneally injected with either beta-naphthoflavone 
      (40 microg/g body weight) or cadmium chloride (10 microg/g body weight), 
      respectively. The chemical data showed that VS6, YTT, and MPM were classified as 
      Class C sediment according to the sediment quality criteria defined by the Hong 
      Kong Environmental Protection Department, indicating the sites were heavily 
      polluted. The exposure of tilapia to the sediment induced hepatic CYP1A1 (VS6 > 
      YTT > MPM > SS6) and MT (VS6 > MPM > YTT > SS6) levels. The induction patterns 
      were comparable to the levels of POPs and metal contamination in the sediment, 
      indicating that the biomarker responses could be used to differentiate low to 
      high levels of contamination among sediment.
FAU - Wong, C K
AU  - Wong CK
AD  - Department of Biology and Institute for Natural Resources and Waste Management, 
      Hong Kong Baptist University, Kowloon Tong.
FAU - Yeung, H Y
AU  - Yeung HY
FAU - Cheung, R Y
AU  - Cheung RY
FAU - Yung, K K
AU  - Yung KK
FAU - Wong, M H
AU  - Wong MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Environ Contam Toxicol
JT  - Archives of environmental contamination and toxicology
JID - 0357245
RN  - 0 (DNA Primers)
RN  - 0 (Metals, Heavy)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants, Chemical)
RN  - 6051-87-2 (beta-Naphthoflavone)
RN  - 9007-49-2 (DNA)
RN  - 9038-94-2 (Metallothionein)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - J6K4F9V3BA (Cadmium Chloride)
SB  - IM
MH  - Animals
MH  - Cadmium Chloride/toxicity
MH  - Cytochrome P-450 CYP1A1/genetics/metabolism
MH  - DNA/analysis
MH  - DNA Primers/chemistry
MH  - Evaluation Studies as Topic
MH  - Geologic Sediments/*chemistry
MH  - Hong Kong
MH  - Liver/enzymology
MH  - Metallothionein/genetics/metabolism
MH  - Metals, Heavy/*analysis
MH  - Polychlorinated Biphenyls/*analysis
MH  - Polycyclic Aromatic Hydrocarbons/*analysis
MH  - RNA, Messenger/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tilapia/metabolism
MH  - Water Pollutants, Chemical/*analysis
MH  - beta-Naphthoflavone/toxicity
EDAT- 2000/04/29 09:00
MHDA- 2000/06/10 09:00
CRDT- 2000/04/29 09:00
PHST- 2000/04/29 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/04/29 09:00 [entrez]
AID - 10.1007/s002449910064 [doi]
PST - ppublish
SO  - Arch Environ Contam Toxicol. 2000 May;38(4):486-93. doi: 10.1007/s002449910064.