PMID- 10788342
OWN - NLM
STAT- MEDLINE
DCOM- 20000616
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 66
IP  - 5
DP  - 2000 May
TI  - Molecular analyses of novel methanotrophic communities in forest soil that 
      oxidize atmospheric methane.
PG  - 1801-8
AB  - Forest and other upland soils are important sinks for atmospheric CH(4), 
      consuming 20 to 60 Tg of CH(4) per year. Consumption of atmospheric CH(4) by soil 
      is a microbiological process. However, little is known about the methanotrophic 
      bacterial community in forest soils. We measured vertical profiles of atmospheric 
      CH(4) oxidation rates in a German forest soil and characterized the 
      methanotrophic populations by PCR and denaturing gradient gel electrophoresis 
      (DGGE) with primer sets targeting the pmoA gene, coding for the alpha subunit of 
      the particulate methane monooxygenase, and the small-subunit rRNA gene (SSU rDNA) 
      of all life. The forest soil was a sink for atmospheric CH(4) in situ and in 
      vitro at all times. In winter, atmospheric CH(4) was oxidized in a well-defined 
      subsurface soil layer (6 to 14 cm deep), whereas in summer, the complete soil 
      core was active (0 cm to 26 cm deep). The content of total extractable DNA was 
      about 10-fold higher in summer than in winter. It decreased with soil depth (0 to 
      28 cm deep) from about 40 to 1 microg DNA per g (dry weight) of soil. The PCR 
      product concentration of SSU rDNA of all life was constant both in winter and in 
      summer. However, the PCR product concentration of pmoA changed with depth and 
      season. pmoA was detected only in soil layers with active CH(4) oxidation, i.e., 
      6 to 16 cm deep in winter and throughout the soil core in summer. The same 
      methanotrophic populations were present in winter and summer. Layers with high 
      CH(4) consumption rates also exhibited more bands of pmoA in DGGE, indicating 
      that high CH(4) oxidation activity was positively correlated with the number of 
      methanotrophic populations present. The pmoA sequences derived from excised DGGE 
      bands were only distantly related to those of known methanotrophs, indicating the 
      existence of unknown methanotrophs involved in atmospheric CH(4) consumption.
FAU - Henckel, T
AU  - Henckel T
AD  - Max-Planck-Institut fur terrestrische Mikrobiologie, Karl-von-Frisch-Strasse, 
      35043 Marburg, Germany.
FAU - Jackel, U
AU  - Jackel U
FAU - Schnell, S
AU  - Schnell S
FAU - Conrad, R
AU  - Conrad R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 1.13.- (Oxygenases)
RN  - EC 1.14.13.25 (methane monooxygenase)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Bacteria/classification/*genetics/*metabolism
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - DNA, Ribosomal/genetics
MH  - Greenhouse Effect
MH  - Methane/*metabolism
MH  - Methylobacterium
MH  - Oxidation-Reduction
MH  - Oxygenases/genetics
MH  - *Phylogeny
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Soil Microbiology
MH  - Trees
PMC - PMC101415
EDAT- 2000/05/02 09:00
MHDA- 2000/06/24 11:00
PMCR- 2000/05/01
CRDT- 2000/05/02 09:00
PHST- 2000/05/02 09:00 [pubmed]
PHST- 2000/06/24 11:00 [medline]
PHST- 2000/05/02 09:00 [entrez]
PHST- 2000/05/01 00:00 [pmc-release]
AID - 1567 [pii]
AID - 10.1128/AEM.66.5.1801-1808.2000 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2000 May;66(5):1801-8. doi: 
      10.1128/AEM.66.5.1801-1808.2000.