PMID- 10788611
OWN - NLM
STAT- MEDLINE
DCOM- 20000630
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 472
IP  - 2-3
DP  - 2000 Apr 28
TI  - Expression of a constitutively active form of phosphatidylinositol 3-kinase 
      inhibits the induction of nitric oxide synthase in human astrocytes.
PG  - 203-7
AB  - The present study underlines the importance of phosphatidylinositol 3-kinase (PI 
      3-kinase) in attenuating the induction of nitric oxide synthase (iNOS) in human 
      astrocytes. Proinflammatory cytokines induced the production of nitric oxide (NO) 
      and the expression of iNOS in human U373MG astrocytoma cells and primary 
      astrocytes. Expression of a catalytically active p110 subunit (p110*) of PI 
      3-kinase but not that of a kinase-deficient mutant of p110 (p110-kd) induced an 
      increase in PI 3-kinase activity and inhibited cytokine-induced production of NO 
      and expression of iNOS. However, expression of p110* had no effect on the 
      activation of NF-kB, suggesting that p110* inhibits the expression of iNOS 
      without inhibiting the activation of NF-kB.
FAU - Pahan, K
AU  - Pahan K
AD  - Department of Oral Biology, University of Nebraska Medical Center College of 
      Dentistry, 40th and Holdrege, Lincoln, NE 68583, USA. kpahan@unmc.edu
FAU - Liu, X
AU  - Liu X
FAU - Wood, C
AU  - Wood C
FAU - Raymond, J R
AU  - Raymond JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Interleukin-1)
RN  - 0 (NF-kappa B)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Animals
MH  - Astrocytes/cytology/drug effects/*enzymology
MH  - Catalysis
MH  - Cells, Cultured
MH  - Enzyme Activation
MH  - Enzyme Induction
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-1/pharmacology
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Nitric Oxide Synthase/*biosynthesis/genetics
MH  - Nitric Oxide Synthase Type II
MH  - Phosphatidylinositol 3-Kinases/*biosynthesis/genetics
MH  - Recombinant Proteins
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2000/05/02 09:00
MHDA- 2000/07/08 11:00
CRDT- 2000/05/02 09:00
PHST- 2000/05/02 09:00 [pubmed]
PHST- 2000/07/08 11:00 [medline]
PHST- 2000/05/02 09:00 [entrez]
AID - S0014-5793(00)01465-4 [pii]
AID - 10.1016/s0014-5793(00)01465-4 [doi]
PST - ppublish
SO  - FEBS Lett. 2000 Apr 28;472(2-3):203-7. doi: 10.1016/s0014-5793(00)01465-4.