PMID- 10789993
OWN - NLM
STAT- MEDLINE
DCOM- 20000512
LR  - 20190921
IS  - 0891-6934 (Print)
IS  - 0891-6934 (Linking)
VI  - 31
IP  - 4
DP  - 1999
TI  - Experimental autoimmune thyroiditis induced by thyroglobulin-pulsed dendritic 
      cells.
PG  - 273-82
AB  - Dendritic cells (DCs), which are the most effective professional 
      antigen-presenting cells (APCs), initiate and regulate immune responses. In this 
      report, we examine the role of DCs in the induction of autoimmune thyroiditis. 
      Experimental autoimmune thyroiditis (EAT) induced by immunization with 
      thyroglobulin (Tg) plus adjuvant is considered to be an animal model of 
      autoimmune thyroiditis, and is categorized as a T cell-mediated autoimmune 
      disease. To examine the contribution of DCs to EAT, naive DCs were purified from 
      high responder B10BR mice and pulsed with various concentrations of porcine Tg 
      (pTg). These pTg-pulsed DCs were transferred without adjuvant to syngenic BIOBR 
      mice to induce EAT. Mice that had received pTg-pulsed DCs showed thyroiditis, and 
      the degree of thyroiditis induced was positively correlated to the amounts of pTg 
      used for the incubation (pulsing) of DCs. The severity of thyroiditis was also 
      correlated to the amounts of anti-pTg IgG2a antibodies and IFN-gamma in the 
      recipient sera, but not to IL-4 or IL-10, indicating that Th1 cells are mainly 
      activated by pTg-pulsed DCs and attributable to the pathogenesis of EAT.
FAU - Watanabe, H
AU  - Watanabe H
AD  - Department of Otorhinolaryngology, Kansai Medical University, Osaka, Japan.
FAU - Inaba, M
AU  - Inaba M
FAU - Adachi, Y
AU  - Adachi Y
FAU - Sugiura, K
AU  - Sugiura K
FAU - Hisha, H
AU  - Hisha H
FAU - Iguchi, T
AU  - Iguchi T
FAU - Ito, T
AU  - Ito T
FAU - Yasumizu, R
AU  - Yasumizu R
FAU - Inaba, K
AU  - Inaba K
FAU - Yamashita, T
AU  - Yamashita T
FAU - Ikehara, S
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Autoimmunity
JT  - Autoimmunity
JID - 8900070
RN  - 0 (Cytokines)
RN  - 9010-34-8 (Thyroglobulin)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cell Transplantation
MH  - Coculture Techniques
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology
MH  - Disease Models, Animal
MH  - Female
MH  - Lymphocyte Activation
MH  - Mice
MH  - Spleen/cytology/immunology
MH  - Thyroglobulin/*immunology
MH  - Thyroiditis, Autoimmune/*chemically induced
EDAT- 2000/05/02 09:00
MHDA- 2000/05/20 09:00
CRDT- 2000/05/02 09:00
PHST- 2000/05/02 09:00 [pubmed]
PHST- 2000/05/20 09:00 [medline]
PHST- 2000/05/02 09:00 [entrez]
AID - 10.3109/08916939908994073 [doi]
PST - ppublish
SO  - Autoimmunity. 1999;31(4):273-82. doi: 10.3109/08916939908994073.