PMID- 10790432
OWN - NLM
STAT- MEDLINE
DCOM- 20000530
LR  - 20231213
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 191
IP  - 9
DP  - 2000 May 1
TI  - Inhibition of CD83 cell surface expression during dendritic cell maturation by 
      interference with nuclear export of CD83 mRNA.
PG  - 1581-90
AB  - Dendritic cells (DCs), nature's adjuvant, must mature to sensitize T cells. 
      However, although the maturation process is essential, it is not yet fully 
      understood at the molecular level. In this study, we investigated the course of 
      expression of the unique hypusine-containing protein eukaryotic initiation factor 
      5A (eIF-5A), which is part of a particular RNA nuclear export pathway, during in 
      vitro generation of human DCs. We show that eIF-5A expression is significantly 
      upregulated during DC maturation. Furthermore, an inhibitor of the hypusine 
      modification, GC7 (N(1)-guanyl-1, 7-diaminoheptane), prevents CD83 surface 
      expression by apparently interfering with nucleocytoplasmic translocation of the 
      CD83 mRNA and, importantly, significantly inhibits DC-mediated T lymphocyte 
      activation. The data presented suggest that CD83 mRNA is transported from the 
      nucleus to the cytoplasm via a specific nuclear export pathway and that hypusine 
      formation appears to be essential for the maturation of functional DCs. 
      Therefore, pharmacological interference with hypusine formation may provide a new 
      possibility to modulate DC function.
FAU - Kruse, M
AU  - Kruse M
AD  - Department of Dermatology, University Erlangen-Nurnberg, D-91054 Erlangen, 
      Germany.
FAU - Rosorius, O
AU  - Rosorius O
FAU - Kratzer, F
AU  - Kratzer F
FAU - Bevec, D
AU  - Bevec D
FAU - Kuhnt, C
AU  - Kuhnt C
FAU - Steinkasserer, A
AU  - Steinkasserer A
FAU - Schuler, G
AU  - Schuler G
FAU - Hauber, J
AU  - Hauber J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, CD)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (N(1)-guanyl-1,7-diaminoheptane)
RN  - 0 (Peptide Initiation Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 3874VXF092 (hypusine)
RN  - 5Z93L87A1R (Guanine)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Antigen Presentation/drug effects
MH  - Antigens, CD
MH  - Biological Transport/drug effects
MH  - Cell Compartmentation/drug effects
MH  - Cell Differentiation
MH  - Cell Nucleus/*metabolism
MH  - Dendritic Cells/*cytology/immunology
MH  - Guanine/analogs & derivatives/pharmacology
MH  - Humans
MH  - Immunoglobulins/*biosynthesis/genetics
MH  - Lymphocyte Activation
MH  - Lysine/*analogs & derivatives/metabolism
MH  - Membrane Glycoproteins/*biosynthesis/genetics
MH  - Peptide Initiation Factors/*metabolism
MH  - Protein Processing, Post-Translational
MH  - RNA, Messenger/*metabolism
MH  - *RNA-Binding Proteins
MH  - T-Lymphocytes/immunology
MH  - Up-Regulation
MH  - Eukaryotic Translation Initiation Factor 5A
MH  - CD83 Antigen
PMC - PMC2213428
EDAT- 2000/05/03 09:00
MHDA- 2000/06/03 09:00
PMCR- 2000/11/01
CRDT- 2000/05/03 09:00
PHST- 2000/05/03 09:00 [pubmed]
PHST- 2000/06/03 09:00 [medline]
PHST- 2000/05/03 09:00 [entrez]
PHST- 2000/11/01 00:00 [pmc-release]
AID - 991614 [pii]
AID - 10.1084/jem.191.9.1581 [doi]
PST - ppublish
SO  - J Exp Med. 2000 May 1;191(9):1581-90. doi: 10.1084/jem.191.9.1581.