PMID- 10790883
OWN - NLM
STAT- MEDLINE
DCOM- 20000710
LR  - 20220330
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 11
IP  - 5
DP  - 2000 Apr 7
TI  - Chondroitinase ABC promotes axonal regeneration of Clarke's neurons after spinal 
      cord injury.
PG  - 1063-7
AB  - We examined whether enzymatic digestion of chondroitin sulfate (CS) promoted the 
      axonal regeneration of neurons in Clarke's nucleus (CN) into a peripheral nerve 
      (PN) graft following injury of the spinal cord. After hemisection at T11, a 
      segment of PN graft was implanted at the lesion site. Either vehicle, 
      brain-derived neurotrophic factor (BDNF) or chondroitinase ABC was applied at the 
      implantation site. The postoperative survival period was 4 weeks. Treatment with 
      vehicle or BDNF did not promote the axonal regeneration of CN neurons into the PN 
      graft. Application of 2.5 unit/ml chondroitinase ABC resulted in a significant 
      increase (12.8%) in the number of regenerated CN neurons. Double labeling with 
      Fluoro-Gold and NADPH-diaphorase histochemistry showed that the regenerated CN 
      neurons did not express nitric oxide synthase (NOS). Our results suggest that CS 
      is inhibitory to the regeneration of CN neurons following injury of the spinal 
      cord.
FAU - Yick, L W
AU  - Yick LW
AD  - Department of Anatomy, Faculty of Medicine, University of Hong Kong, Hong Kong.
FAU - Wu, W
AU  - Wu W
FAU - So, K F
AU  - So KF
FAU - Yip, H K
AU  - Yip HK
FAU - Shum, D K
AU  - Shum DK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Stilbamidines)
RN  - 9007-28-7 (Chondroitin Sulfates)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.6.99.1 (NADPH Dehydrogenase)
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - Animals
MH  - Axons/*drug effects/metabolism/ultrastructure
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Chondroitin ABC Lyase/*pharmacology
MH  - Chondroitin Sulfates/metabolism
MH  - Denervation/adverse effects
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Fluorescent Dyes
MH  - Immunohistochemistry
MH  - NADPH Dehydrogenase/analysis
MH  - Nerve Regeneration/*drug effects/physiology
MH  - Nitric Oxide Synthase/metabolism
MH  - Peripheral Nerves/drug effects/metabolism/transplantation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/*drug effects/*pathology/physiopathology
MH  - Spinal Cord Injuries/*drug therapy/pathology/physiopathology
MH  - Spinocerebellar Tracts/*drug effects/*injuries/pathology
MH  - *Stilbamidines
EDAT- 2000/05/03 09:00
MHDA- 2000/07/15 11:00
CRDT- 2000/05/03 09:00
PHST- 2000/05/03 09:00 [pubmed]
PHST- 2000/07/15 11:00 [medline]
PHST- 2000/05/03 09:00 [entrez]
AID - 10.1097/00001756-200004070-00032 [doi]
PST - ppublish
SO  - Neuroreport. 2000 Apr 7;11(5):1063-7. doi: 10.1097/00001756-200004070-00032.