PMID- 10791829
OWN - NLM
STAT- MEDLINE
DCOM- 20000626
LR  - 20240109
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 43
IP  - 2
DP  - 2000 Apr
TI  - Molecular cytogenetic analysis of supernumerary heterochromatic segments in Rumex 
      acetosa.
PG  - 391-7
AB  - The dioecious plant Rumex acetosa shows intraspecific karyotype variation, caused 
      by supernumerary heterochromatic segments or DAPI (4',6-diamidino-2 
      phenylindole)-bands at the ends of the short arms of three pairs of autosomes. A 
      DNA sequence (RAE730) specific to the supernumerary heterochromatic segments was 
      cloned and sequenced. RAE730 was about 730 bp and AT-rich (71% AT-content). Using 
      fluorescence in situ hybridization (FISH), RAE730 was localized in the 
      supernumerary DAPI-positive heterochromatic segments on several mitotic 
      chromosomes and chromocenters in interphase nuclei, but not in the DAPI-bands of 
      Y or B chromosomes. RAE730 was tandemly arranged in the genome, and the copy 
      number varied between plants from 40000 to 304000 copies per 2C, corresponding to 
      the relative amount of supernumerary heterochromatic segments per genome. These 
      results indicate that the karyotype variation caused by the supernumerary 
      heterochromatic segment was generated by amplification or reduction of the tandem 
      repeats of RAE730.
FAU - Shibata, F
AU  - Shibata F
AD  - Institute of Biology, Faculty of Science, Ehime University, Matsuyama, Japan. 
      shibataf@sci.ehime-u.ac.jp
FAU - Hizume, M
AU  - Hizume M
FAU - Kuroki, Y
AU  - Kuroki Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chromosome Banding
MH  - Diploidy
MH  - *Genes, Plant
MH  - Genome, Plant
MH  - Heterochromatin/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Molecular Sequence Data
MH  - Polygonaceae/*genetics
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 2000/05/03 09:00
MHDA- 2000/07/06 11:00
CRDT- 2000/05/03 09:00
PHST- 2000/05/03 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/05/03 09:00 [entrez]
AID - 10.1139/g99-128 [doi]
PST - ppublish
SO  - Genome. 2000 Apr;43(2):391-7. doi: 10.1139/g99-128.