PMID- 10792628
OWN - NLM
STAT- MEDLINE
DCOM- 20000601
LR  - 20071114
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 57
IP  - 5
DP  - 2000 May
TI  - Glomerular type IV collagen in patients with diabetic nephropathy with and 
      without additional glomerular disease.
PG  - 2084-92
AB  - BACKGROUND: Type IV collagen is a constituent of mesangial matrix and is 
      increased in amount in many forms of glomerular injury. METHODS: We performed 
      renal biopsies in patients who (1) were donating a kidney to a relative (LRD, N = 
      6), (2) had diabetic glomerulopathy with or without nephrosclerosis (DM, N = 6), 
      or (3) had diabetic glomerulopathy with a superimposed glomerular lesion (DM+, N 
      = 5). Glomerular collagen alpha2(IV) and control glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH) mRNAs were measured, and the former correlated with 
      clinical and morphological data to assess its usefulness in reflecting glomerular 
      injury. RESULTS: Collagen alpha2(IV) mRNA levels were lowest in LRD (2.9 +/- 0.6 
      attomol/glomerulus), higher in DM (5.9 +/- 1.6, P = 0.05), and highest in DM+ 
      (12.7 +/- 2.8 attm/glomerulus, P < 0.05 vs. LRD and vs. DM). Control GAPDH mRNA 
      levels were not significantly different (P > 0.05). Levels of proteinuria, serum 
      creatinine, and glomerular size did not correlate with collagen alpha2(IV) mRNA 
      levels. The fractional mesangial area and the fractional mesangial area occupied 
      by type IV collagen were higher in both diabetic groups than in LRD (P < 10-6), 
      but the intensity of type IV collagen staining in the diabetic patients was 
      significantly less than that seen in the LRD (P < 0.01). In DM+ patients, 
      extramesangial type IV collagen was present. Fractional mesangial area and 
      glomerular collagen alpha2(IV) mRNA levels correlated (r = 0.45, P < 0.05). 
      CONCLUSION: These data are consistent with a view of diabetic nephropathy as a 
      lesion of increased alpha2 type IV collagen transcription, increased total amount 
      of collagen present, but decreased mesangial density relative to other matrix 
      molecules. These data further demonstrate that glomerular injury superimposed on 
      diabetic nephropathy contributes to additional structural damage by inducing 
      increased synthesis of type IV collagen at extramesangial sites.
FAU - Adler, S G
AU  - Adler SG
AD  - Harbor-UCLA Research and Education Institute, Torrance, CA 90509, USA. 
      sadler@rei.edu
FAU - Feld, S
AU  - Feld S
FAU - Striker, L
AU  - Striker L
FAU - Striker, G
AU  - Striker G
FAU - LaPage, J
AU  - LaPage J
FAU - Esposito, C
AU  - Esposito C
FAU - Aboulhosn, J
AU  - Aboulhosn J
FAU - Barba, L
AU  - Barba L
FAU - Cha, D R
AU  - Cha DR
FAU - Nast, C C
AU  - Nast CC
LA  - eng
GR  - MO1 RR00425/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (RNA, Messenger)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adult
MH  - Collagen/analysis/*genetics
MH  - Diabetic Nephropathies/*metabolism/pathology
MH  - Humans
MH  - Hypertrophy
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Kidney Glomerulus/*metabolism/pathology
MH  - Middle Aged
MH  - RNA, Messenger/*analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2000/05/03 09:00
MHDA- 2000/06/03 09:00
CRDT- 2000/05/03 09:00
PHST- 2000/05/03 09:00 [pubmed]
PHST- 2000/06/03 09:00 [medline]
PHST- 2000/05/03 09:00 [entrez]
AID - S0085-2538(15)46961-3 [pii]
AID - 10.1046/j.1523-1755.2000.00058.x [doi]
PST - ppublish
SO  - Kidney Int. 2000 May;57(5):2084-92. doi: 10.1046/j.1523-1755.2000.00058.x.