PMID- 10793352
OWN - NLM
STAT- MEDLINE
DCOM- 20000614
LR  - 20221007
IS  - 0194-5998 (Print)
IS  - 0194-5998 (Linking)
VI  - 122
IP  - 5
DP  - 2000 May
TI  - Expression of matrix metalloproteinases and tissue inhibitor of 
      metalloproteinases in laryngeal and pharyngeal squamous cell carcinoma: A 
      quantitative analysis.
PG  - 712-6
AB  - Squamous cell carcinomas of the head and neck are known for their aggressive 
      growth and propensity to metastasize. Invasion is facilitated by matrix 
      metalloproteineases (MMPs). Tissue inhibitors of MMPs (TIMPs) negatively regulate 
      MMP activity. MMP and TIMP expression in head and neck squamous cell carcinomas 
      was determined by quantitative reverse transcriptase polymerase chain reaction 
      (qRT-PCR). qRT-PCR allows measurement of several mRNAs from as little as 4 microg 
      of total cellular RNA. We measured MMP-1, MMP-2, MMP-9, and TIMP-1 expression in 
      8 specimens of primary tumors and adjacent normal tissue. MMP-1 was overexpressed 
      in 6 of 8 tumors, and MMP-9 was overexpressed in 4 of 7 tumors. MMP-2 was 
      expressed in 3 of 8 tumors and 3 of 8 normal samples. TIMP-1 was expressed in all 
      specimens. This work demonstrates that qRT-PCR can be used to examine expression 
      of specific mRNAs in clinical specimens. Therefore this method provides another 
      tool for the molecular analysis of tumors.
FAU - Magary, S P
AU  - Magary SP
AD  - Department of Otolaryngology, University of Florida College of Medicine, 
      Gainesville 32610-0264, USA.
FAU - Ryan, M W
AU  - Ryan MW
FAU - Tarnuzzer, R W
AU  - Tarnuzzer RW
FAU - Kornberg, L
AU  - Kornberg L
LA  - eng
PT  - Journal Article
PL  - England
TA  - Otolaryngol Head Neck Surg
JT  - Otolaryngology--head and neck surgery : official journal of American Academy of 
      Otolaryngology-Head and Neck Surgery
JID - 8508176
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*enzymology
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*chemistry
MH  - Male
MH  - Matrix Metalloproteinase 1/analysis
MH  - Matrix Metalloproteinase 2/analysis
MH  - Matrix Metalloproteinase 9/analysis
MH  - Matrix Metalloproteinases/*analysis
MH  - Middle Aged
MH  - Pharyngeal Neoplasms/*chemistry
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinase-1/*analysis
EDAT- 2000/05/04 09:00
MHDA- 2000/06/17 09:00
CRDT- 2000/05/04 09:00
PHST- 2000/05/04 09:00 [pubmed]
PHST- 2000/06/17 09:00 [medline]
PHST- 2000/05/04 09:00 [entrez]
AID - S0194599800547871 [pii]
AID - 10.1016/S0194-5998(00)70202-6 [doi]
PST - ppublish
SO  - Otolaryngol Head Neck Surg. 2000 May;122(5):712-6. doi: 
      10.1016/S0194-5998(00)70202-6.