PMID- 10794151
OWN - NLM
STAT- MEDLINE
DCOM- 20000615
LR  - 20061115
IS  - 0723-2020 (Print)
IS  - 0723-2020 (Linking)
VI  - 22
IP  - 4
DP  - 1999 Dec
TI  - Characterization of microbial communities of biofilters by phospholipid fatty 
      acid analysis and rRNA targeted oligonucleotide probes.
PG  - 626-34
AB  - The microbial community of a biofilter for waste gas treatment of an animal 
      rendering plant was characterized by the analyses of the phospholipid fatty acids 
      (PLFAs) of the filter material. For these analyses five samples of one filter 
      were taken in intervals between one and two months. The main components of the 
      PLFA profiles were straight chain saturated, monounsaturated and cyclopropyl 
      fatty acids. Terminally branched and 10-methyl branched fatty acids were present 
      in minor amounts. The structure and succession of the microbial community was 
      interpreted by the presence and quantitative changes of diagnostic fatty acids. 
      The stability of diagnostic fatty acids in relation to varying incubation 
      parameters was tested for a number of bacterial isolates from biofilters 
      representing different phylogenetic branches. For two samples, the data from the 
      PLFA-analyses were compared with data obtained by hybridization with 
      fluorescently labeled, rRNA-targeted oligonucleotide probes specific for the 
      alpha-, beta- and gamma-subclass of the Proteobacteria, the Actinobacteria 
      (Firmicutes with high G+C content) and the Firmicutes with low G+C content. These 
      data indicated a dominating number of Proteobacteria (54% and 35% of DAPI-stained 
      cells), in which the gamma-Proteobacteria represented the main fraction. 
      Actinobacteria were detected in minor amounts, the number of Firmicutes with low 
      G+C content was near the detection limit of the method. About half of the cells 
      detected with a probe specific for Bacteria did not hybridize with the probes 
      specific for the alpha-, beta- and gamma subclass of the Proteobacteria and the 
      two subgroups of the Firmicutes. The results of both methods, the fluorescence in 
      situ hybridization (FISH) and the PLFA analyses corresponded well and were best 
      suited to confirm and complement each other.
FAU - von Keitz, V
AU  - von Keitz V
AD  - Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universitat Osnabruck, 
      Germany.
FAU - Schramm, A
AU  - Schramm A
FAU - Altendorf, K
AU  - Altendorf K
FAU - Lipski, A
AU  - Lipski A
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (Air Pollutants)
RN  - 0 (Fatty Acids)
RN  - 0 (Hazardous Waste)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Phospholipids)
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - Air Pollutants/metabolism
MH  - Bacteria/chemistry/*classification/genetics/isolation & purification
MH  - Colony Count, Microbial
MH  - *Ecosystem
MH  - Fatty Acids/*analysis
MH  - Filtration/*instrumentation
MH  - Hazardous Waste
MH  - *In Situ Hybridization, Fluorescence
MH  - Oligonucleotide Probes
MH  - Phospholipids/chemistry
MH  - RNA, Ribosomal/genetics
EDAT- 2000/05/04 09:00
MHDA- 2000/06/17 09:00
CRDT- 2000/05/04 09:00
PHST- 2000/05/04 09:00 [pubmed]
PHST- 2000/06/17 09:00 [medline]
PHST- 2000/05/04 09:00 [entrez]
AID - S0723-2020(99)80016-2 [pii]
AID - 10.1016/S0723-2020(99)80016-2 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 1999 Dec;22(4):626-34. doi: 10.1016/S0723-2020(99)80016-2.