PMID- 10794162
OWN - NLM
STAT- MEDLINE
DCOM- 20000511
LR  - 20190620
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 90
IP  - 2
DP  - 2000 Apr 25
TI  - Numeric chromosomal abnormalities in small lymphocytic and transformed large cell 
      lymphomas detected by fluorescence in situ hybridization of fine-needle 
      aspiration biopsies.
PG  - 126-32
AB  - BACKGROUND: Chromosomal abnormalities in some lymphomas are associated with a 
      poor prognosis. Trisomy 12 and deletions of chromosome 13 have been described in 
      small lymphocytic lymphoma (SLL). To determine whether chromosomal aberrations 
      could be detected by fluorescence in situ hybridization (FISH) on fine-needle 
      aspiration biopsies (FNABs), we analyzed and compared specimens from seven 
      patients with SLL and nine patients with a history of SLL that had transformed to 
      large cell lymphoma. METHODS: DNA probes specific to chromosome 12 and the 
      chromosome 13/RB-1 gene locus were used for in situ hybridization on interphase 
      cell nuclei. The cytologic features, ploidy, proliferation index, and 
      conventional cytogenetic analysis findings were correlated with the FISH results. 
      RESULTS: Trisomy 12 was detected in 2 (29%) of the 7 cases of SLL and 2 (22%) of 
      the 9 cases of transformed large cell lymphoma. Deletion of chromosome 13/RB-1 
      was present in 3 (43%) of 7 cases of SLL and 3 (33%) of 9 cases of transformed 
      large cell lymphoma. CONCLUSIONS: This study showed that interphase cytogenetic 
      analysis by FISH is feasible on FNAB specimens. Trisomy 12 and deletions of 
      chromosome 13/RB-1 were present in some cases of SLL and transformed large cell 
      lymphoma, and the presence of these chromosomal abnormalities did not correlate 
      with transformation to a higher grade of lymphoma.
FAU - Caraway, N P
AU  - Caraway NP
AD  - Department of Pathology, The University of Texas M.D. Anderson Cancer Center, 
      Houston 77030, USA.
FAU - Du, Y
AU  - Du Y
FAU - Zhang, H Z
AU  - Zhang HZ
FAU - Hayes, K
AU  - Hayes K
FAU - Glassman, A B
AU  - Glassman AB
FAU - Katz, R L
AU  - Katz RL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Needle
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/pathology
MH  - Lymphoma, Large B-Cell, Diffuse/*genetics/pathology
MH  - Male
MH  - Middle Aged
EDAT- 2000/05/04 09:00
MHDA- 2000/05/16 09:00
CRDT- 2000/05/04 09:00
PHST- 2000/05/04 09:00 [pubmed]
PHST- 2000/05/16 09:00 [medline]
PHST- 2000/05/04 09:00 [entrez]
AID - 10.1002/(SICI)1097-0142(20000425)90:2<126::AID-CNCR8>3.0.CO;2-9 [pii]
AID - 10.1002/(sici)1097-0142(20000425)90:2<126::aid-cncr8>3.0.co;2-9 [doi]
PST - ppublish
SO  - Cancer. 2000 Apr 25;90(2):126-32. doi: 
      10.1002/(sici)1097-0142(20000425)90:2<126::aid-cncr8>3.0.co;2-9.