PMID- 10794573
OWN - NLM
STAT- MEDLINE
DCOM- 20000623
LR  - 20190831
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 108
IP  - 8
DP  - 2000 Mar
TI  - Intranuclear arrangement of human chromosome 12 correlates to large-scale 
      replication domains.
PG  - 514-22
AB  - The intranuclear arrangement of human chromosome 12 in G0(G1) nuclei from human 
      myeloid leukemia HL60 cells was analyzed by multicolor fluorescence in situ 
      hybridization (FISH) using band-specific cosmid clones as probes. Pairs of 
      differently colored cosmids were detected on paraformaldehyde-fixed HL60 nuclei, 
      and their relative positions, internal or peripheral, in individual nuclei were 
      scored. Our results suggest that the intranuclear arrangement of human chromosome 
      12 is not random. Some chromosomal domains, including the centromere, were 
      located in the periphery of the nucleus, while other domains, including the 
      telomeres, were positioned in the internal areas of the nucleus in GO(G1) cells. 
      Based on the replication banding patterns of metaphase spreads, human chromosome 
      12 was divided roughly into five large domains. Interestingly, the clones in late 
      replicating domains were preferentially localized in the nuclear periphery, 
      whereas clones in early replicating domains were arranged in the internal areas 
      of the nuclei. The DNA replication timing of each cosmid determined by FISH-based 
      assay did not reflect the replication bands, but an overall profile of the 
      replication timing was relatively correlated with these domains on chromosome 12. 
      These results suggest that the intranuclear arrangement of a human chromosome is 
      correlated with the large-scale replication domains, even before DNA replication.
FAU - Nogami, M
AU  - Nogami M
AD  - Laboratory of Biological Chemistry, Faculty of Bioresources, Mie University, Tsu, 
      Japan.
FAU - Nogami, O
AU  - Nogami O
FAU - Kagotani, K
AU  - Kagotani K
FAU - Okumura, M
AU  - Okumura M
FAU - Taguchi, H
AU  - Taguchi H
FAU - Ikemura, T
AU  - Ikemura T
FAU - Okumura, K
AU  - Okumura K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
SB  - IM
MH  - Cell Nucleus/*ultrastructure
MH  - *Chromosomes, Human, Pair 12
MH  - Cosmids
MH  - DNA Replication/*genetics
MH  - G1 Phase
MH  - HL-60 Cells
MH  - Humans
MH  - Resting Phase, Cell Cycle
EDAT- 2000/05/04 09:00
MHDA- 2000/07/06 11:00
CRDT- 2000/05/04 09:00
PHST- 2000/05/04 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/05/04 09:00 [entrez]
AID - 10.1007/s004120050403 [doi]
PST - ppublish
SO  - Chromosoma. 2000 Mar;108(8):514-22. doi: 10.1007/s004120050403.