PMID- 10795740
OWN - NLM
STAT- MEDLINE
DCOM- 20000523
LR  - 20230413
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 12
IP  - 4
DP  - 2000 Apr
TI  - The distinct roles of TRAF2 and RIP in IKK activation by TNF-R1: TRAF2 recruits 
      IKK to TNF-R1 while RIP mediates IKK activation.
PG  - 419-29
AB  - The death domain kinase RIP and the TNF receptor-associated factor 2 (TRAF2) are 
      essential effectors in TNF signaling. To understand the mechanism by which RIP 
      and TRAF2 regulate TNF-induced activation of the transcription factor NF-kappaB, 
      we investigated their respective roles in TNF-R1-mediated IKK activation using 
      both RIP-/- and TRAF2-/- fibroblasts. We found that TNF-R1-mediated IKK 
      activation requires both RIP and TRAF2 proteins. Although TRAF2 or RIP can be 
      independently recruited to the TNF-R1 complex, neither one of them alone is 
      capable of transducing the TNF signal that leads to IKK activation. Moreover, we 
      demonstrated that IKK is recruited to the TNF-R1 complex through TRAF2 upon TNF 
      treatment and that IKK activation requires the presence of RIP in the same 
      complex.
FAU - Devin, A
AU  - Devin A
AD  - Department of Cell and Cancer Biology, Medicine Branch, Division of Clinical 
      Sciences, National Cancer Institute, National Institutes of Health, Bethesda, 
      Maryland 20892, USA.
FAU - Cook, A
AU  - Cook A
FAU - Lin, Y
AU  - Lin Y
FAU - Rodriguez, Y
AU  - Rodriguez Y
FAU - Kelliher, M
AU  - Kelliher M
FAU - Liu, Z
AU  - Liu Z
LA  - eng
GR  - R01 GM061298/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Antigens, CD)
RN  - 0 (Macromolecular Substances)
RN  - 0 (NF-kappa B)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (RIPK1 protein, human)
RN  - EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.10 (CHUK protein, human)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
RN  - EC 2.7.11.10 (IKBKB protein, human)
RN  - EC 2.7.11.10 (IKBKE protein, human)
SB  - IM
MH  - Antigens, CD/analysis/chemistry/*physiology
MH  - Apoptosis/*physiology
MH  - Cells, Cultured
MH  - Enzyme Activation
MH  - Fibroblasts/cytology
MH  - Humans
MH  - I-kappa B Kinase
MH  - Macromolecular Substances
MH  - NF-kappa B/*physiology
MH  - Protein Serine-Threonine Kinases/chemistry/*physiology
MH  - Proteins/*physiology
MH  - Receptor-Interacting Protein Serine-Threonine Kinases
MH  - Receptors, Tumor Necrosis Factor/analysis/chemistry/*physiology
MH  - Receptors, Tumor Necrosis Factor, Type I
MH  - Recombinant Fusion Proteins/physiology
MH  - Signal Transduction/*physiology
MH  - TNF Receptor-Associated Factor 2
MH  - *Transcription, Genetic
MH  - Transfection
EDAT- 2000/05/05 09:00
MHDA- 2000/06/08 09:00
CRDT- 2000/05/05 09:00
PHST- 2000/05/05 09:00 [pubmed]
PHST- 2000/06/08 09:00 [medline]
PHST- 2000/05/05 09:00 [entrez]
AID - S1074-7613(00)80194-6 [pii]
AID - 10.1016/s1074-7613(00)80194-6 [doi]
PST - ppublish
SO  - Immunity. 2000 Apr;12(4):419-29. doi: 10.1016/s1074-7613(00)80194-6.