PMID- 10797080
OWN - NLM
STAT- MEDLINE
DCOM- 20000811
LR  - 20190513
IS  - 0305-7453 (Print)
IS  - 0305-7453 (Linking)
VI  - 45
IP  - 5
DP  - 2000 May
TI  - Epidemiological studies of large resistance plasmids in Haemophilus.
PG  - 599-604
AB  - The distribution of large conjugative Haemophilus influenzae plasmids in the 
      nasopharyngeal haemophili of a group of people and in a large collection of 541 
      H. influenzae type b (Hib) isolates was studied. A newly developed PCR-based 
      assay was used to detect the plasmids. The target sequences were chosen from 
      sequence analysis of part of p1056, a large multiresistance plasmid isolated from 
      a clinical Hib isolate, 1056. Fifty-nine per cent of people were found to carry 
      beta-lactamase-positive (beta-lac(+)), ampicillin-resistant (ampR) haemophili 
      with detectable plasmid sequences. Of these, 83% were in Haemophilus 
      parainfluenzae and 17% were in H. influenzae. In the collection of 541 Hib, 
      antibiotic resistance [beta-lac(+)ampR, beta-lac(+)ampR plus tetracycline 
      resistance (tetR) or tetR] was highly correlated with large plasmids. It was 
      found that 2.3% of the isolates contained large cryptic plasmids (i.e. these 
      isolates were susceptible to antibiotics). The distribution of plasmids between 
      invasive and carried Hib did not differ significantly (25 of 245 and 23 of 276, 
      respectively). Isolates with large plasmids occur at high frequency in the 
      nasopharynx of the normal human population and consist of two populations in Hib, 
      one associated with specific antibiotic resistance traits and the other cryptic. 
      These plasmids do not appear to influence the invasiveness of Hib.
FAU - Leaves, N I
AU  - Leaves NI
AD  - Public Health Laboratory, John Radcliffe Hospital, Oxford.
FAU - Dimopoulou, I
AU  - Dimopoulou I
FAU - Hayes, I
AU  - Hayes I
FAU - Kerridge, S
AU  - Kerridge S
FAU - Falla, T
AU  - Falla T
FAU - Secka, O
AU  - Secka O
FAU - Adegbola, R A
AU  - Adegbola RA
FAU - Slack, M P
AU  - Slack MP
FAU - Peto, T E
AU  - Peto TE
FAU - Crook, D W
AU  - Crook DW
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Antimicrob Chemother
JT  - The Journal of antimicrobial chemotherapy
JID - 7513617
RN  - 0 (Anti-Bacterial Agents)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Adult
MH  - Ampicillin Resistance/*genetics
MH  - Anti-Bacterial Agents/pharmacology
MH  - Carrier State/epidemiology/microbiology
MH  - Child
MH  - Haemophilus Infections/*epidemiology/microbiology
MH  - Haemophilus influenzae/*drug effects/genetics/isolation & purification
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Nasopharynx/microbiology
MH  - Plasmids/*genetics
MH  - Polymerase Chain Reaction
MH  - Tetracycline Resistance/*genetics
MH  - beta-Lactamases/metabolism
EDAT- 2000/05/08 09:00
MHDA- 2000/08/19 11:00
CRDT- 2000/05/08 09:00
PHST- 2000/05/08 09:00 [pubmed]
PHST- 2000/08/19 11:00 [medline]
PHST- 2000/05/08 09:00 [entrez]
AID - 10.1093/jac/45.5.599 [doi]
PST - ppublish
SO  - J Antimicrob Chemother. 2000 May;45(5):599-604. doi: 10.1093/jac/45.5.599.