PMID- 10797531
OWN - NLM
STAT- MEDLINE
DCOM- 20000602
LR  - 20131121
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 60
IP  - 3
DP  - 2000 May 1
TI  - Laminin modulates neuritogenesis of developing rat retinal ganglion cells through 
      a protein kinase C-dependent pathway.
PG  - 291-301
AB  - Dissociated cells from rat retinae (P2-P21) were cultured to investigate 
      interactions between brain-derived neurotrophic factor (BDNF), various substrates 
      (poly-L-lysine, collagen, and laminin), and protein kinases upon the 
      neuritogenesis of retinal ganglion cells (RGCs). We found that BDNF-promoted 
      neuritogenesis was enhanced by forskolin in RGCs from rats at P2-P21 plated on 
      either poly-L-lysine or collagen. In contrast, in cultures with a laminin 
      substrate, the enhancer effect of forskolin was observed only in RGCs taken from 
      the retina of rats at P2-P6. Laminin blocked the enhancement of BDNF-induced RGCs 
      neuritogenesis by forskolin, in RGCs from either P14 or P21, and induced a 
      tenfold increase of protein kinase C (PKC) activity compared to poly-L-lysine. 
      This blockade was reverted with a selective PKC inhibitor and was reproduced in 
      poly-L-lysine cultures of P14-P21 RGCs with a PKC activator. Because axotomized 
      RGCs need both BDNF and forskolin to regenerate, we suggest that laminin can 
      hinder this effect by simultaneous PKC activation according to a developmentally 
      regulated pattern. We further propose a model of interaction in the optic 
      pathways triggered by BDNF, forskolin, and laminin that may be useful in 
      elucidating some of the biological effects seen with regenerating axons.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - de Ary-Pires, R
AU  - de Ary-Pires R
AD  - Instituto de Biofisica da UFRJ, Rio de Janeiro, Brazil.
FAU - Linden, R
AU  - Linden R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Laminin)
RN  - 1F7A44V6OU (Colforsin)
RN  - 25104-18-1 (Polylysine)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Animals
MH  - Biotransformation/physiology
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - Colforsin/pharmacology
MH  - Collagen/metabolism
MH  - Laminin/metabolism/*physiology
MH  - Neurites/*physiology
MH  - Polylysine/metabolism
MH  - Protein Kinase C/*physiology
MH  - Rats
MH  - Retinal Ganglion Cells/*physiology
EDAT- 2000/05/08 09:00
MHDA- 2000/06/10 09:00
CRDT- 2000/05/08 09:00
PHST- 2000/05/08 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/05/08 09:00 [entrez]
AID - 10.1002/(SICI)1097-4547(20000501)60:3<291::AID-JNR3>3.0.CO;2-Y [pii]
AID - 10.1002/(SICI)1097-4547(20000501)60:3<291::AID-JNR3>3.0.CO;2-Y [doi]
PST - ppublish
SO  - J Neurosci Res. 2000 May 1;60(3):291-301. doi: 
      10.1002/(SICI)1097-4547(20000501)60:3<291::AID-JNR3>3.0.CO;2-Y.