PMID- 10797543
OWN - NLM
STAT- MEDLINE
DCOM- 20000602
LR  - 20220409
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 60
IP  - 3
DP  - 2000 May 1
TI  - Expression of alpha(2)-macroglobulin receptor/low density lipoprotein 
      receptor-related protein (LRP) in rat microglial cells.
PG  - 401-11
AB  - Low density lipoprotein receptor-related protein (LRP) participates in the uptake 
      and degradation of several ligands implicated in neuronal pathophysiology 
      including apolipoprotein E (apoE), activated alpha(2) -macroglobulin (alpha(2)M*) 
      and beta-amyloid precursor protein (APP). The receptor is expressed in a variety 
      of tissues. In the brain LRP is present in pyramidal-type neurons in cortical and 
      hippocampal regions and in astrocytes that are activated as a result of injury or 
      neoplasmic transformation. As LRP is expressed in the monocyte/macrophage cell 
      system, we were interested in examining whether LRP is expressed in microglia. We 
      isolated glial cells from the brain of neonatal rats and LRP was immunodetected 
      both in microglial cells and in astrocytes expressing glial fibrillar acidic 
      protein (GFAP). Microglial cells were able to bind and internalize LRP-specific 
      ligand, alpha(2)M*. The internalization was inhibitable by RAP, with a Kd of 1.7 
      nM. The expression of LRP was up-regulated by dexamethasone, and down-regulated 
      by lipopolysaccharide (LPS), gamma interferon (IFN-gamma) or a combination of 
      both. LRP was less sensitive to dexamethasone in activated astrocytes than in 
      microglia. We provided the first analysis of LRP expression and regulation in 
      microglia. Our results open the possibility that microglial cells could be 
      related to the participation of LRP and its ligands in different 
      pathophysiological states in brain.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Marzolo, M P
AU  - Marzolo MP
AD  - Departamento de Biologia Celular y Molecular, Facultad de Ciencias Biologicas, 
      Pontificia Universidad Catolica de Chile, Santiago, Chile.
FAU - von Bernhardi, R
AU  - von Bernhardi R
FAU - Bu, G
AU  - Bu G
FAU - Inestrosa, N C
AU  - Inestrosa NC
LA  - eng
GR  - NS37525/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Glucocorticoids)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Low Density Lipoprotein Receptor-Related Protein-1)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, LDL)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Alzheimer Disease/metabolism/pathology
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Dexamethasone/pharmacology
MH  - Down-Regulation/drug effects
MH  - Fluorescent Antibody Technique
MH  - Glucocorticoids/pharmacology
MH  - Immunohistochemistry
MH  - Interferon-gamma/pharmacology
MH  - Kinetics
MH  - Ligands
MH  - Lipopolysaccharides/pharmacology
MH  - Low Density Lipoprotein Receptor-Related Protein-1
MH  - Microglia/*metabolism
MH  - Neuroglia/metabolism
MH  - Precipitin Tests
MH  - Rats
MH  - Receptors, Immunologic/*biosynthesis
MH  - Receptors, LDL/*biosynthesis
MH  - Up-Regulation/drug effects
EDAT- 2000/05/08 09:00
MHDA- 2000/06/10 09:00
CRDT- 2000/05/08 09:00
PHST- 2000/05/08 09:00 [pubmed]
PHST- 2000/06/10 09:00 [medline]
PHST- 2000/05/08 09:00 [entrez]
AID - 10.1002/(SICI)1097-4547(20000501)60:3<401::AID-JNR15>3.0.CO;2-L [pii]
AID - 10.1002/(SICI)1097-4547(20000501)60:3<401::AID-JNR15>3.0.CO;2-L [doi]
PST - ppublish
SO  - J Neurosci Res. 2000 May 1;60(3):401-11. doi: 
      10.1002/(SICI)1097-4547(20000501)60:3<401::AID-JNR15>3.0.CO;2-L.