PMID- 10799277
OWN - NLM
STAT- MEDLINE
DCOM- 20000829
LR  - 20131121
IS  - 0882-4010 (Print)
IS  - 0882-4010 (Linking)
VI  - 28
IP  - 5
DP  - 2000 May
TI  - An RNA helicase, RHIV -1, induced by porcine reproductive and respiratory 
      syndrome virus (PRRSV) is mapped on porcine chromosome 10q13.
PG  - 267-78
AB  - The impact of porcine reproductive and respiratory syndrome virus (PRRSV) 
      infection on porcine alveolar macrophages (Mo) was examined by differential 
      display reverse transcription PCR (DDRT-PCR). A PRRSV-induced expressed gene tag 
      (EST) was used to isolate and identify a single cDNA clone from a library 
      prepared from porcine peripheral blood. Rapid amplification of cDNA ends (RACE) 
      was employed to clone a 1.5 kb fragment at the 5' end of the mRNA. DNA sequencing 
      identified an open reading frame (ORF) of 2820 bp. Deduced amino acid sequence 
      revealed the eight conserved domains characteristic of the DEAD/H box protein 
      superfamily. The putative porcine RNA helicase induced by virus (RHIV -1) showed 
      84% amino acid similarity to human retinoic acid-induced gene (RIG-I). Porcine 
      RHIV -1 transcripts were ubiquitously expressed in various pig tissues, while in 
      PRRSV-infected pigs, higher expression was observed in several tissues persistent 
      for PRRSV. These data indicate the association of PRRSV genome replication with 
      enhaced host cell RNA helicase gene expression. Finally, the RHIV -1 gene was 
      localized on porcine chromosome 10q13 between markers SSC25A02 and SWR334 via 
      somatic cell panel and radiation hybrid (RH) mapping strategies.
CI  - Copyright 2000 Academic Press.
FAU - Zhang, X
AU  - Zhang X
AD  - Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN 
      55108, USA.
FAU - Wang, C
AU  - Wang C
FAU - Schook, L B
AU  - Schook LB
FAU - Hawken, R J
AU  - Hawken RJ
FAU - Rutherford, M S
AU  - Rutherford MS
LA  - eng
SI  - GENBANK/AF181119
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.4.13 (RNA Helicases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - *Chromosome Mapping
MH  - DNA, Complementary/blood/genetics
MH  - Macrophages, Alveolar/virology
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory Syndrome/metabolism/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/metabolism
MH  - RNA Helicases/*genetics/metabolism
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Swine
MH  - Virus Replication
EDAT- 2000/05/09 09:00
MHDA- 2000/09/02 11:01
CRDT- 2000/05/09 09:00
PHST- 2000/05/09 09:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 2000/05/09 09:00 [entrez]
AID - S0882-4010(99)90349-2 [pii]
AID - 10.1006/mpat.1999.0349 [doi]
PST - ppublish
SO  - Microb Pathog. 2000 May;28(5):267-78. doi: 10.1006/mpat.1999.0349.