PMID- 10800159
OWN - NLM
STAT- MEDLINE
DCOM- 20000830
LR  - 20061115
IS  - 0390-6078 (Print)
IS  - 0390-6078 (Linking)
VI  - 85
IP  - 5
DP  - 2000 May
TI  - Human herpesvirus 6 in hematologic diseases in China.
PG  - 458-63
AB  - BACKGROUND AND OBJECTIVE: The prevalence and pathogenic role of human herpesvirus 
      6 (HHV-6) in various benign and malignant hematologic diseases remain largely 
      unknown. The aim of this study was to search for a possible involvement of HHV-6 
      in the pathogenesis of hematologic diseases. DESIGN AND METHODS: The presence of 
      HHV-6 DNA sequences was examined by polymerase chain reaction (PCR) in bone 
      marrow mononuclear cells from 241 patients with benign and malignant hematologic 
      diseases in China. Platelet-associated immunoglobulin (PAIg) of 66 idiopathic 
      thrombocytopenic purpura (ITP) patients was measured by competitive enzyme-linked 
      immunosorbent assay. The presence of HHV-6 DNA in sera from 31 ITP patients was 
      examined by PCR. Paired serum samples from 19 ITP patients were analyzed for 
      anti-HHV-6 IgG titers using an indirect immunofluorescence assay. RESULTS: HHV-6 
      DNA was detected in 41% and 37.5% of ITP and acute leukemia patients 
      respectively, but in only 6.7% of patients with iron deficiency anemia. HHV-6 
      positivity for ITP patients with excessive PAIgG was significantly higher than in 
      patients with a normal level of PAIgG. HHV-6 DNA was not detected in any of the 
      serum samples from ITP patients. None of the 19 cases of ITP showed a significant 
      increase in anti-HHV-6 antibody titers during the convalescent phase compared 
      with the onset phase. INTERPRETATION AND CONCLUSIONS: Our results indicate that 
      HHV-6 infection might be associated with excessive PAIgG in some cases of ITP, 
      and that the virus persists in a latent state. The pathogenic role of HHV-6 in 
      ITP needs to be confirmed by further investigations.
FAU - Ma, X T
AU  - Ma XT
AD  - National Laboratory of Experimental Hematology, Institute of Hematology and Blood 
      Diseases Hospital, Peking Union Medical College, Chinese Academy of Medical 
      Sciences, Tianjin, China.
FAU - Song, Y H
AU  - Song YH
FAU - Lu, D M
AU  - Lu DM
FAU - Mu, G F
AU  - Mu GF
FAU - Li, G
AU  - Li G
FAU - Ji, L X
AU  - Ji LX
FAU - Yang, R C
AU  - Yang RC
FAU - Wu, K F
AU  - Wu KF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
RN  - 0 (Autoantibodies)
RN  - 0 (DNA, Viral)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - Anemia, Iron-Deficiency/virology
MH  - Autoantibodies/blood
MH  - Blood Platelets/immunology
MH  - Bone Marrow Cells/metabolism/virology
MH  - China/epidemiology
MH  - DNA, Viral/blood
MH  - Female
MH  - Hematologic Diseases/epidemiology/*virology
MH  - Herpesviridae Infections/complications
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Immunoglobulin G/blood
MH  - Leukemia/virology
MH  - Leukocytes, Mononuclear/virology
MH  - Male
MH  - Purpura, Thrombocytopenic, Idiopathic/virology
EDAT- 2000/05/09 09:00
MHDA- 2000/09/02 11:01
CRDT- 2000/05/09 09:00
PHST- 2000/05/09 09:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 2000/05/09 09:00 [entrez]
PST - ppublish
SO  - Haematologica. 2000 May;85(5):458-63.