PMID- 10802344
OWN - NLM
STAT- MEDLINE
DCOM- 20000714
LR  - 20190915
IS  - 0168-0102 (Print)
IS  - 0168-0102 (Linking)
VI  - 37
IP  - 1
DP  - 2000 May
TI  - BDNF rapidly induces aspartate release from cultured CNS neurons.
PG  - 59-65
AB  - We investigated the effects of brain-derived neurotrophic factor (BDNF) on 
      aspartate release from cultured cerebellar neurons. This release occurred within 
      1 min after the addition of 100 ng/ml BDNF. The amount of aspartate released was 
      less than that of glutamate. Aspartate release induced by BDNF was rapid and 
      transient, as in the case of glutamate. Although high potassium evoked the 
      release of both excitatory (glutamate and aspartate) and inhibitory (GABA and 
      glycine) amino acid transmitters, BDNF only induced glutamate and aspartate 
      release. BDNF-induced aspartate release was completely blocked by pretreatment 
      with K252a or TrkB-IgG. The aspartate release induced by BDNF was not dependent 
      on the extracellular Ca(2+), but required intracellular Ca(2+) mobilization. 
      These results showed that BDNF may be involved in excitatory transmission using 
      aspartate as well as glutamate through TrkB-mediated signaling in cerebellum.
FAU - Numakawa, T
AU  - Numakawa T
AD  - Division of Protein Biosynthesis, Institute for Protein Research, Osaka 
      University, 3-2 Yamadaoka, Suita, Osaka, Japan.
FAU - Takei, N
AU  - Takei N
FAU - Hatanaka, H
AU  - Hatanaka H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Aspartic Acid/*drug effects/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism/*pharmacology
MH  - Calcium/metabolism
MH  - Cells, Cultured
MH  - Cerebellar Cortex/cytology/*drug effects/*metabolism
MH  - Cerebral Cortex/cytology/drug effects/metabolism
MH  - Glutamic Acid/drug effects/metabolism
MH  - Hippocampus/cytology/drug effects/metabolism
MH  - Immunohistochemistry
MH  - Intracellular Fluid/chemistry/metabolism
MH  - Microtubule-Associated Proteins/drug effects/metabolism
MH  - Neostriatum/cytology/drug effects/metabolism
MH  - Neurons/cytology/*drug effects/*metabolism
MH  - Rats
MH  - Receptor, trkB/*drug effects/*metabolism
MH  - Time Factors
EDAT- 2000/05/10 09:00
MHDA- 2000/07/25 11:00
CRDT- 2000/05/10 09:00
PHST- 2000/05/10 09:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/05/10 09:00 [entrez]
AID - S0168-0102(00)00103-6 [pii]
AID - 10.1016/s0168-0102(00)00103-6 [doi]
PST - ppublish
SO  - Neurosci Res. 2000 May;37(1):59-65. doi: 10.1016/s0168-0102(00)00103-6.