PMID- 10802388
OWN - NLM
STAT- MEDLINE
DCOM- 20000620
LR  - 20190819
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 115
IP  - 2
DP  - 2000 May 19
TI  - Expression of heat shock protein 60 in human proximal tubule cells exposed to 
      heat, sodium arsenite and CdCl(2).
PG  - 127-36
AB  - The expression of hsp 60 mRNA and protein were determined in human proximal 
      tubule cells (HPT) exposed to lethal and sub-lethal concentrations of Cd(2+) 
      under both acute and extended conditions of exposure. It was demonstrated that 
      HPT cells exhibited the classic heat shock response when subjected to a physical 
      (heat) or chemical stress (sodium arsenite). Heat stress, elevated temperature at 
      42.5 degrees C for 1 h, caused an increase in both hsp 60 mRNA and protein 
      following removal of the stress. Similar results were obtained when the cells 
      were subjected to a classic chemical stress of exposure to 100 microM sodium 
      arsenite for 4 h. Acute exposure of HPT cells to 53.4 microM CdCl(2) for 4 h also 
      resulted in an increase in hsp 60 mRNA and protein following removal of the 
      metal. An extended exposure to Cd(2+) was modeled by treating the cells 
      continuously with Cd(2+) at both lethal and sub-lethal levels over a 16-day time 
      course. It was demonstrated that chronic exposure to Cd(2+) failed to increase 
      either hsp 60 mRNA or protein expression in HPT cells, even at concentrations of 
      Cd(2+) that were lethal to the cells during the time course. In fact, hsp 60 
      protein levels were decreased compared to controls at lethal levels of Cd(2+) 
      exposure. These findings suggest that hsp 60 expression may have two distinct 
      roles when the human proximal tubule cell is exposed to Cd(2+). A protective role 
      through hsp 60 induction when the proximal tubule cell is acutely exposed to 
      Cd(2+) and a deleterious role when hsp 60 protein is down-regulated during 
      extended exposure to Cd(2+).
FAU - Somji, S
AU  - Somji S
AD  - Robert C. Byrd Health Sciences Center, Department of Pathology, West Virginia 
      University, PO Box 9203, Morgantown, WV 26506, USA.
FAU - Todd, J H
AU  - Todd JH
FAU - Sens, M A
AU  - Sens MA
FAU - Garrett, S H
AU  - Garrett SH
FAU - Sens, D A
AU  - Sens DA
LA  - eng
GR  - ESO7687/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Arsenites)
RN  - 0 (Chaperonin 60)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sodium Compounds)
RN  - 48OVY2OC72 (sodium arsenite)
RN  - J6K4F9V3BA (Cadmium Chloride)
SB  - IM
MH  - Arsenites/pharmacology
MH  - Cadmium Chloride/pharmacology/*toxicity
MH  - Cell Line
MH  - Chaperonin 60/*biosynthesis/genetics
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation
MH  - Hot Temperature
MH  - Humans
MH  - Kidney Tubules, Proximal/*drug effects/metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Sodium Compounds/pharmacology
EDAT- 2000/05/10 09:00
MHDA- 2000/06/24 11:00
CRDT- 2000/05/10 09:00
PHST- 2000/05/10 09:00 [pubmed]
PHST- 2000/06/24 11:00 [medline]
PHST- 2000/05/10 09:00 [entrez]
AID - S0378-4274(00)00183-1 [pii]
AID - 10.1016/s0378-4274(00)00183-1 [doi]
PST - ppublish
SO  - Toxicol Lett. 2000 May 19;115(2):127-36. doi: 10.1016/s0378-4274(00)00183-1.