PMID- 10802738
OWN - NLM
STAT- MEDLINE
DCOM- 20000525
LR  - 20131121
IS  - 1072-8368 (Print)
IS  - 1072-8368 (Linking)
VI  - 7
IP  - 5
DP  - 2000 May
TI  - RmlC, the third enzyme of dTDP-L-rhamnose pathway, is a new class of epimerase.
PG  - 398-402
AB  - Deoxythymidine diphosphate (dTDP)-L-rhamnose is the precursor of L-rhamnose, a 
      saccharide required for the virulence of some pathogenic bacteria. 
      dTDP-L-rhamnose is synthesized from glucose-1-phosphate and deoxythymidine 
      triphosphate (dTTP) via a pathway involving four distinct enzymes. This pathway 
      does not exist in humans and the enzymes involved in dTDP-L-rhamnose synthesis 
      are potential targets for the design of new therapeutic agents. Here, the crystal 
      structure of dTDP-6-deoxy-D-xylo-4-hexulose 3,5 epimerase (RmlC, EC5.1.3.13) from 
      Salmonella enterica serovar Typhimurium was determined. The third enzyme of the 
      rhamnose biosynthetic pathway, RmlC epimerizes at two carbon centers, the 3 and 5 
      positions of the sugar ring. The structure was determined by multiwavelength 
      anomalous diffraction to a resolution of 2.17 A. RmlC is a dimer and each monomer 
      is formed mainly from two beta-sheets arranged in a beta-sandwich. The structure 
      of a dTDP-phenol-RmlC complex shows the substrate-binding site to be located 
      between the two beta-sheets; this site is formed from residues of both monomers. 
      Sequence alignments of other RmlC enzymes confirm that this region is very highly 
      conserved. The enzyme is distinct structurally from other epimerases known and 
      thus, is the first example of a new class of carbohydrate epimerase.
FAU - Giraud, M F
AU  - Giraud MF
AD  - Centre for Biomolecular Sciences, North Haugh, The University, St. Andrews, Fife 
      KY16 9ST, Scotland.
FAU - Leonard, G A
AU  - Leonard GA
FAU - Field, R A
AU  - Field RA
FAU - Berlind, C
AU  - Berlind C
FAU - Naismith, J H
AU  - Naismith JH
LA  - eng
SI  - PDB/1DZR
SI  - PDB/1DZT
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Nat Struct Biol
JT  - Nature structural biology
JID - 9421566
RN  - 0 (Nucleoside Diphosphate Sugars)
RN  - 0 (Thymine Nucleotides)
RN  - 0 (phenol deoxythymidine diphosphate)
RN  - 2147-59-3 (thymidine diphosphate rhamnose)
RN  - 339NCG44TV (Phenol)
RN  - EC 5.1.3.- (Carbohydrate Epimerases)
RN  - EC 5.1.3.13 (dTDP-4-ketorhamnose 3,5-epimerase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Carbohydrate Epimerases/*chemistry/*classification/metabolism
MH  - Conserved Sequence
MH  - Crystallography, X-Ray
MH  - Dimerization
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Nucleoside Diphosphate Sugars/chemistry/*metabolism
MH  - Phenol/chemistry/metabolism
MH  - Protein Binding
MH  - Protein Structure, Secondary
MH  - Salmonella typhimurium/*enzymology
MH  - Sequence Alignment
MH  - Thymine Nucleotides/chemistry/*metabolism
EDAT- 2000/05/10 09:00
MHDA- 2000/06/08 09:00
CRDT- 2000/05/10 09:00
PHST- 2000/05/10 09:00 [pubmed]
PHST- 2000/06/08 09:00 [medline]
PHST- 2000/05/10 09:00 [entrez]
AID - 10.1038/75178 [doi]
PST - ppublish
SO  - Nat Struct Biol. 2000 May;7(5):398-402. doi: 10.1038/75178.