PMID- 10804293
OWN - NLM
STAT- MEDLINE
DCOM- 20000629
LR  - 20231112
IS  - 0910-5050 (Print)
IS  - 1876-4673 (Electronic)
IS  - 0910-5050 (Linking)
VI  - 91
IP  - 4
DP  - 2000 Apr
TI  - Photodynamic inactivation with acridine orange on a multidrug-resistant mouse 
      osteosarcoma cell line.
PG  - 439-45
AB  - Overcoming multidrug resistance (MDR) is an urgent issue to improve the prognosis 
      of osteosarcoma patients. In this study, we undertook to clarify the effect of 
      photodynamic therapy (PDT) with acridine orange (AO) on the MDR mouse 
      osteosarcoma (MOS / ADR1) cell line, by comparing the outcome with the effect on 
      a chemosensitive osteosarcoma (MOS) cell line. Cultured cells of MOS and MOS / 
      ADR1 cell lines were exposed to AO at various concentrations for various times, 
      followed by long- or short-term (10 or 1 min) illumination with blue light (466.5 
      nm) for excitation. Living cells were counted by means of the trypan blue 
      exclusion test. The results showed that AO rapidly bound to DNA, RNA and 
      lysosomes of living MOS and MOS / ADR1 cells and also that most tumor cells in 
      both cell lines died rapidly (viability ratio to untreated cells: 1/1000) within 
      48 h under conditions of continuous or 15-min flash exposure to AO at 
      concentrations above 1.0 microg/ml plus 10-min illumination with blue light. Even 
      after flash exposure to AO at concentrations above 1.0 microg/ml plus 1-min 
      illumination, the viability of MOS/ADR1 cells decreased to a viability ratio of 
      less than 1/ 1000 within 72 h. Based on these results, we concluded that AO with 
      photo-excitation has a strong cytocidal effect, not only on chemosensitive mouse 
      osteosarcoma cells, but also on MDR mouse osteosarcoma cells. These results 
      suggested that photodynamic therapy with AO may be a new approach to treating MDR 
      human osteosarcomas.
FAU - Kusuzaki, K
AU  - Kusuzaki K
AD  - Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine, 
      Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan. kusu@basic.kpu-m.ac.jp
FAU - Minami, G
AU  - Minami G
FAU - Takeshita, H
AU  - Takeshita H
FAU - Murata, H
AU  - Murata H
FAU - Hashiguchi, S
AU  - Hashiguchi S
FAU - Nozaki, T
AU  - Nozaki T
FAU - Ashihara, T
AU  - Ashihara T
FAU - Hirasawa, Y
AU  - Hirasawa Y
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/pharmacokinetics/*pharmacology
MH  - Animals
MH  - Bone Neoplasms/*drug therapy/pathology
MH  - Drug Resistance, Multiple
MH  - Drug Resistance, Neoplasm
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Osteosarcoma/*drug therapy/pathology
MH  - *Photochemotherapy
MH  - Ploidies
MH  - Tumor Cells, Cultured
PMC - PMC5926471
EDAT- 2000/05/11 09:00
MHDA- 2000/07/06 11:00
PMCR- 2000/04/01
CRDT- 2000/05/11 09:00
PHST- 2000/05/11 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/05/11 09:00 [entrez]
PHST- 2000/04/01 00:00 [pmc-release]
AID - CAE439 [pii]
AID - 10.1111/j.1349-7006.2000.tb00964.x [doi]
PST - ppublish
SO  - Jpn J Cancer Res. 2000 Apr;91(4):439-45. doi: 10.1111/j.1349-7006.2000.tb00964.x.