PMID- 10805080
OWN - NLM
STAT- MEDLINE
DCOM- 20000717
LR  - 20231213
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 300
IP  - 1
DP  - 2000 Apr
TI  - Acute-phase response and circadian expression of connexin26 are not altered in 
      connexin32-deficient mouse liver.
PG  - 111-7
AB  - In mouse hepatocytes, the gap junctional proteins connexin32 (Cx32) and 
      connexin26 (Cx26) are expressed in the same gap junctional plaque. Expression of 
      the major Cx32 protein is downregulated during liver regeneration and 
      cholestasis. Here we have analyzed the acute-phase response (after experimental 
      inflammation) and circadian connexin expression in Cx32-deficient and wild-type 
      mouse liver. Acute-phase response was triggered by intraperitoneal injection of 
      lipopolysaccharide (LPS). Injection of recombinant mouse interleukin-1beta 
      (mIL-1beta), mIL-6 or tumor necrosis factor alpha (mTNF-alpha) had no 
      inflammatory effect. Northern blot analysis of positive and negative acute-phase 
      transcripts following stimulation with cytokine or LPS revealed no difference 
      between Cx32-deficient livers and wild-type controls, suggesting that loss of the 
      Cx32 gene had no effect on experimental liver inflammation. Actin, 
      beta-fibrinogen and Cx26 transcripts were increased after endotoxin stimulation. 
      Under conditions of hepatic acute-phase response, Cx32 transcripts were not 
      detected in LPS-treated livers of wild-type mice. Immunoblot analysis of proteins 
      from inflamed wild-type livers indicated a strongly diminished amount of Cx32 
      protein, whereas the level of Cx26 protein was increased. Although 
      intraperitoneal injection of mIL-1, mIL-6 as well as mTNF-alpha did not induce an 
      acute-phase response, Cx32 protein expression was diminished, suggesting that 
      post-transcriptional downregulation of Cx32 preceded the acute-phase response. 
      Northern blot hybridization of RNA from wild-type and Cx32-deficient mouse liver 
      revealed a similar circadian regulation of Cx26 and GAPDH transcripts with 
      maximal expression around 2 p.m. and a minimum after midnight.
FAU - Temme, A
AU  - Temme A
AD  - Abteilung Molekulargenetik, Institut fur Genetik, Universitat Bonn, Germany.
FAU - Ott, T
AU  - Ott T
FAU - Haberberger, T
AU  - Haberberger T
FAU - Traub, O
AU  - Traub O
FAU - Willecke, K
AU  - Willecke K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Actins)
RN  - 0 (Albumins)
RN  - 0 (Connexins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 127120-53-0 (Connexin 26)
RN  - 9001-32-5 (Fibrinogen)
SB  - IM
MH  - Actins/genetics
MH  - Acute-Phase Reaction/*metabolism
MH  - Albumins/genetics
MH  - Animals
MH  - Blotting, Northern
MH  - Circadian Rhythm/*physiology
MH  - Connexin 26
MH  - Connexins/*genetics
MH  - Fibrinogen/genetics
MH  - Fluorescent Antibody Technique, Indirect
MH  - Gap Junctions/metabolism
MH  - Gene Expression/physiology
MH  - Liver/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger/analysis
MH  - RNA, Ribosomal, 18S/metabolism
MH  - Gap Junction beta-1 Protein
EDAT- 2000/05/11 09:00
MHDA- 2000/07/25 11:00
CRDT- 2000/05/11 09:00
PHST- 2000/05/11 09:00 [pubmed]
PHST- 2000/07/25 11:00 [medline]
PHST- 2000/05/11 09:00 [entrez]
AID - 10.1007/s004410000177 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2000 Apr;300(1):111-7. doi: 10.1007/s004410000177.