PMID- 10805933
OWN - NLM
STAT- MEDLINE
DCOM- 20000627
LR  - 20191103
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 40
IP  - 2
DP  - 2000 Jun 1
TI  - Susceptibility of human herpesvirus 6 to antiviral compounds by flow cytometry 
      analysis.
PG  - 135-40
AB  - BACKGROUND: The emergence of human herpesvirus 6 (HHV-6) as a human pathogen led 
      to the possibility of specific therapy against HHV-6 and the development of 
      standardized susceptibility assays of HHV-6 to antivirals. METHODS: We have 
      developed a flow cytometry method to analyze the multiplication of the HST strain 
      of human herpesvirus 6 (HHV-6) variant B in vitro using monoclonal antibodies 
      specific to virus proteins. This method was subsequently used to determine the 
      sensitivity of HST multiplication in MT4 cells to four antiviral compounds of 
      three different classes: acyclovir (ACV) and ganciclovir (GCV), two acyclic 
      guanosine analogs; cedofovir (CDV), an acyclic nucleoside phosphonate; and 
      phosphonoformic acid (PFA), a pyrophosphate analog. RESULTS: The 50% inhibitory 
      concentrations (IC(50)) of ACV, GCV, CDV, and PFA determined by flow cytometry 
      assay were 25.3, 6.4, 0.95, and 6.0 microM, respectively (5.7, 1.6, 0.3, and 1.8 
      microg/ml, respectively). These data together with the results of cytotoxicity 
      assays confirmed the high efficiency and selectivity of CDV and PFA against HHV-6 
      B in vitro, suggested by previous results. CONCLUSIONS: Our flow cytometric assay 
      appeared as a reproducible specific method to characterize HHV-6 susceptibility 
      to antiviral compounds. It can be considered as a convenient alternative to the 
      other immunologic and DNA hybridization assays used for that purpose.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Manichanh, C
AU  - Manichanh C
AD  - Laboratoire de Virologie, CERVI, Groupe Hospitalier Pitie-Salpetriere, Paris, 
      France.
FAU - Grenot, P
AU  - Grenot P
FAU - Gautheret-Dejean, A
AU  - Gautheret-Dejean A
FAU - Debre, P
AU  - Debre P
FAU - Huraux, J M
AU  - Huraux JM
FAU - Agut, H
AU  - Agut H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Surface)
RN  - 0 (Antigens, Viral)
RN  - 0 (Antiviral Agents)
RN  - 0 (Organophosphonates)
RN  - 0 (Organophosphorus Compounds)
RN  - 364P9RVW4X (Foscarnet)
RN  - 8J337D1HZY (Cytosine)
RN  - JIL713Q00N (Cidofovir)
RN  - P9G3CKZ4P5 (Ganciclovir)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, Surface/analysis/immunology
MH  - Antigens, Viral/analysis/immunology
MH  - Antiviral Agents/*pharmacology
MH  - Cidofovir
MH  - Cytosine/*analogs & derivatives/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Flow Cytometry/methods/standards
MH  - Foscarnet/pharmacology
MH  - Ganciclovir/pharmacology
MH  - Herpesviridae Infections/*drug therapy
MH  - Herpesvirus 6, Human/drug effects/*growth & development/immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Leukocytes, Mononuclear/*cytology/*virology
MH  - *Organophosphonates
MH  - Organophosphorus Compounds/*pharmacology
MH  - Reproducibility of Results
EDAT- 2000/05/11 09:00
MHDA- 2000/07/06 11:00
CRDT- 2000/05/11 09:00
PHST- 2000/05/11 09:00 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 2000/05/11 09:00 [entrez]
AID - 10.1002/(SICI)1097-0320(20000601)40:2<135::AID-CYTO7>3.0.CO;2-H [pii]
AID - 10.1002/(sici)1097-0320(20000601)40:2<135::aid-cyto7>3.0.co;2-h [doi]
PST - ppublish
SO  - Cytometry. 2000 Jun 1;40(2):135-40. doi: 
      10.1002/(sici)1097-0320(20000601)40:2<135::aid-cyto7>3.0.co;2-h.