PMID- 10806066
OWN - NLM
STAT- MEDLINE
DCOM- 20000802
LR  - 20090723
IS  - 1047-8477 (Print)
IS  - 1047-8477 (Linking)
VI  - 129
IP  - 2-3
DP  - 2000 Apr
TI  - The dynamics of yeast telomeres and silencing proteins through the cell cycle.
PG  - 159-74
AB  - Genes integrated near the telomeres of budding yeast have a variegated pattern of 
      gene repression that is mediated by the silent information regulatory proteins 
      Sir2p, Sir3p, and Sir4p. Immunolocalization and fluorescence in situ 
      hybridization (FISH) reveal 6-10 perinuclear foci in which silencing proteins and 
      subtelomeric sequences colocalize, suggesting that these are sites of 
      Sir-mediated repression. Telomeres lacking subtelomeric repeat elements and the 
      silent mating locus, HML, also localize to the periphery of the nucleus. 
      Conditions that disrupt telomere proximal repression disrupt the focal staining 
      pattern of Sir proteins, but not necessarily the localization of telomeric DNA. 
      To monitor the telomere-associated pools of heterochromatin-binding proteins (Sir 
      and Rap1 proteins) during mitotic cell division, we have performed 
      immunofluorescence and telomeric FISH on populations of yeast cells synchronously 
      traversing the cell cycle. We observe a partial release of Rap1p from telomeres 
      in late G2/M, although telomeres appear to stay clustered during G2-phase and 
      throughout mitosis. A partial release of Sir3p and Sir4p during mitosis also 
      occurs. This is not observed upon HU arrest, although other types of DNA damage 
      cause a dramatic relocalization of Sir and Rap1 proteins. The observed cell cycle 
      dynamics were confirmed by direct epifluorescence of a GFP-Rap1p fusion. Using 
      live GFP fluorescence we show that the diffuse mitotic distribution of GFP-Rap1p 
      is restored to the interphase pattern of foci in early G1-phase.
CI  - Copyright 2000 Academic Press.
FAU - Laroche, T
AU  - Laroche T
AD  - Swiss Institute for Experimental Cancer Research (ISREC), Chemin des Boveresses 
      155, Epalinges, CH-1066, Switzerland.
FAU - Martin, S G
AU  - Martin SG
FAU - Tsai-Pflugfelder, M
AU  - Tsai-Pflugfelder M
FAU - Gasser, S M
AU  - Gasser SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Struct Biol
JT  - Journal of structural biology
JID - 9011206
RN  - 0 (Fungal Proteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SIR1 protein, S cerevisiae)
RN  - 0 (SIR3 protein, S cerevisiae)
RN  - 0 (SIR4 protein, S cerevisiae)
RN  - 0 (Silent Information Regulator Proteins, Saccharomyces cerevisiae)
RN  - 0 (Trans-Activators)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Cell Cycle/*genetics
MH  - Cell Nucleus/genetics/physiology
MH  - Fungal Proteins/*metabolism
MH  - Genes, Regulator
MH  - Genes, Reporter
MH  - Green Fluorescent Proteins
MH  - Luminescent Proteins/genetics
MH  - Mitosis
MH  - Recombinant Fusion Proteins/metabolism
MH  - Saccharomyces cerevisiae/*cytology/*genetics
MH  - *Silent Information Regulator Proteins, Saccharomyces cerevisiae
MH  - Telomere/genetics/*physiology
MH  - Trans-Activators/*metabolism
EDAT- 2000/05/12 09:00
MHDA- 2000/08/06 11:00
CRDT- 2000/05/12 09:00
PHST- 2000/05/12 09:00 [pubmed]
PHST- 2000/08/06 11:00 [medline]
PHST- 2000/05/12 09:00 [entrez]
AID - S1047-8477(00)94240-0 [pii]
AID - 10.1006/jsbi.2000.4240 [doi]
PST - ppublish
SO  - J Struct Biol. 2000 Apr;129(2-3):159-74. doi: 10.1006/jsbi.2000.4240.