PMID- 10806226
OWN - NLM
STAT- MEDLINE
DCOM- 20000823
LR  - 20240109
IS  - 0032-0889 (Print)
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Linking)
VI  - 123
IP  - 1
DP  - 2000 May
TI  - Cloning, developmental, and tissue-specific expression of sucrose:sucrose 
      1-fructosyl transferase from Taraxacum officinale. Fructan localization in roots.
PG  - 71-80
AB  - Sucrose:sucrose 1-fructosyl transferase (1-SST) is the key enzyme initiating 
      fructan synthesis in Asteraceae. Using reverse transcriptase-PCR, we isolated the 
      cDNA for 1-SST from Taraxacum officinale. The cDNA-derived amino acid sequence 
      showed very high homology to other Asteracean 1-SSTs (Cichorium intybus 86%, 
      Cynara scolymus 82%, Helianthus tuberosus 80%), but homology to 1-SST from Allium 
      cepa (46%) and Aspergillus foetidus (18%) was much lower. Fructan concentrations, 
      1-SST activities, 1-SST protein, and mRNA concentrations were compared in 
      different organs during vegetative and generative development of T. officinale 
      plants. Expression of 1-SST was abundant in young roots but very low in leaves. 
      1-SST was also expressed at the flowering stages in roots, stalks, and 
      receptacles. A good correlation was found between northern and western blots 
      showing transcriptional regulation of 1-SST. At the pre-flowering stage, 1-SST 
      mRNA concentrations and 1-SST activities were higher in the root phloem than in 
      the xylem, resulting in the higher fructan concentrations in the phloem. Fructan 
      localization studies indicated that fructan is preferentially stored in phloem 
      parenchyma cells in the vicinity of the secondary sieve tube elements. However, 
      inulin-like crystals occasionally appeared in xylem vessels.
FAU - Van den Ende, W
AU  - Van den Ende W
AD  - Department of Biology, Botany Institute, K.U., Kardinaal Mercierlaan 92, B-3001 
      Heverlee, Belgium. wim.vandenede@bio.kuleven.ac.be
FAU - Michiels, A
AU  - Michiels A
FAU - Van Wonterghem, D
AU  - Van Wonterghem D
FAU - Vergauwen, R
AU  - Vergauwen R
FAU - Van Laere, A
AU  - Van Laere A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (DNA, Complementary)
RN  - 0 (Fructans)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.4.1.- (Hexosyltransferases)
RN  - EC 2.4.1.9 (inulosucrase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - DNA, Complementary
MH  - Fructans/*metabolism
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Plant
MH  - Hexosyltransferases/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - *Plant Proteins
MH  - Plant Roots/metabolism
MH  - Plants/*enzymology/genetics/metabolism
MH  - RNA, Messenger/genetics
MH  - Sequence Homology, Amino Acid
PMC - PMC58983
EDAT- 2000/05/12 09:00
MHDA- 2000/08/29 11:01
PMCR- 2001/05/01
CRDT- 2000/05/12 09:00
PHST- 2000/05/12 09:00 [pubmed]
PHST- 2000/08/29 11:01 [medline]
PHST- 2000/05/12 09:00 [entrez]
PHST- 2001/05/01 00:00 [pmc-release]
AID - 5988 [pii]
AID - 10.1104/pp.123.1.71 [doi]
PST - ppublish
SO  - Plant Physiol. 2000 May;123(1):71-80. doi: 10.1104/pp.123.1.71.